FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962632

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962632
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473248481
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1360300.032.039621
TCGGAAG1511550.028.852632
CGGAAGA1762450.024.8397983
TATGCCG804450.013.13135745-49
CGGCTAT881350.012.02491630-34
CGTATGC939000.011.33312245-49
CTCGTAT894300.011.06981740-44
TCTCGTA944800.010.48886840-44
AGAGCAC4461750.010.3537437
GATCGGA852900.010.1551611
ATGCCGT1105400.09.81463745-49
AGCACAC4784700.09.786599
ACCGGCT1103150.09.73462630-34
GAGCACA4902550.09.5040448
GAAGAGC4989350.09.3793655
GCCGTCT1175100.09.16007650-54
GTCACCG1220800.08.89263325-29
ATCTCGT1185550.08.50313540-44
CACCGGC1334000.08.10327930-34
TGCCGTC1138950.08.09491645-49
Produced by FastQC (version 0.11.5)