FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962633

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962633
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences473248481
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1704650.025.5180389
GAGCGTC2094750.020.8397438
ATCGGAA2275900.019.4407621
TCGGAAG2426250.018.276432
AGAGCGT2454100.017.9388317
CGGAAGA2656650.016.7454663
AAGAGCG2874600.015.5067936
CGCCGTA989100.010.625648555-59
TCGCCGT1140750.09.24994555-59
GCCGTAT1249000.08.66648855-59
GTCGCCG1299600.08.18291555-59
GAAGAGC5928600.08.0925325
CGTATCA1438850.07.61473760-64
TGGTCGC1423900.07.591962350-54
GTGGTCG1572900.06.96495750-54
CCGTATC1377500.06.724482555-59
GCGTCGT1643550.06.36987810-14
AGTACGT1744050.06.216337735-39
GGTCGCC1480950.06.198113450-54
CGTCGTG1696850.06.188592410-14
Produced by FastQC (version 0.11.5)