FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467258603
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC12342560.264148373529251Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3877800.049.9419149
GAGCGTC4289450.045.2233478
AGAGCGT4684800.041.5508657
ATCGGAA4856800.040.7432671
TCGGAAG4914850.040.1284982
CGGAAGA5190100.037.998893
AAGAGCG5231250.037.5823446
GAAGAGC8241950.024.262915
CGCCGTA2153400.018.3937555-59
GGAAGAG11847500.017.1784574
TCGCCGT2344700.016.91529555-59
GCCGTAT2380950.016.69550955-59
GTCGCCG2503750.015.80022455-59
CCGTATC2498600.015.65002455-59
TGGTCGC2551750.015.59430350-54
CGTATCA2556300.015.4964460-64
GGTCGCC2639450.014.87293850-54
GTGGTCG2687300.014.855185550-54
TCTCGGT3133650.012.90740145-49
CCGTGTA3181300.012.69181235-39
Produced by FastQC (version 0.11.5)