FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467920594
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC13098430.27992847863413334Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3527900.057.924169
GAGCGTC3946800.051.845748
AGAGCGT4303450.047.7292447
ATCGGAA4443700.046.8101161
TCGGAAG4514950.045.966132
CGGAAGA4790300.043.263243
AAGAGCG4858100.042.5626376
GAAGAGC7898150.026.5379335
CGCCGTA2169350.019.23939355-59
GGAAGAG11436800.018.6624054
TCGCCGT2373350.017.61317355-59
GCCGTAT2439450.017.26726355-59
TGGTCGC2520550.016.70175450-54
GTCGCCG2504100.016.666355-59
CCGTATC2540850.016.17589255-59
CGTATCA2591950.016.15159460-64
GTGGTCG2646250.015.95223750-54
GGTCGCC2608400.015.796247550-54
CCGTGTA3008000.014.25475235-39
TCTCGGT3080850.013.90371545-49
Produced by FastQC (version 0.11.5)