FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962645

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962645
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468380539
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC6676320.14254050807179244Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1508550.068.1742869
GAGCGTC1896500.054.469298
ATCGGAA2049800.050.9212151
AGAGCGT2205000.047.0335247
TCGGAAG2221200.047.0088462
CGGAAGA2468200.042.36133
AAGAGCG2597800.040.192986
CGCCGTA1020300.020.39912455-59
GAAGAGC5716700.018.8187225
TCGCCGT1152450.018.17445255-59
GCCGTAT1263350.016.64101855-59
GTCGCCG1260950.016.58991455-59
GTACGTC1322000.015.73293130-34
TGGTCGC1367000.015.53083750-54
TACGTCC1417850.014.83499930-34
CCGTATC1412750.014.75191755-59
GGTCGCC1435300.014.61806550-54
GCGTCGT1439950.014.49731910-14
GTGGTCG1485500.014.35926250-54
CGTATCA1480950.014.15752760-64
Produced by FastQC (version 0.11.5)