FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences490672421
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTCAGAGCCGTGTAGATCTC9546380.1945570933158275Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG1781950.081.558399
GAGCGTC2119750.068.7753758
ATCGGAA2338700.062.849761
TCGGAAG2509200.058.8094332
AGAGCGT2538350.057.5605747
CGGAAGA2763500.053.451873
AAGAGCG3013900.048.9618266
TCCGATC485350.025.5289573
GAAGAGC6217050.024.2197255
CGCCGTA1382050.021.74290555-59
TCGCCGT1480100.020.29959555-59
GTCGCCG1505750.019.68700455-59
TTCCGAT675650.018.902512
CCGATCT644950.018.6604144
GCCGTAT1669050.018.1779155-59
TGGTCGC1733700.017.43634850-54
GGTCGCC1710700.017.23512850-54
GCGTCGT1727700.017.2263810-14
CGTCGTG1777100.016.84134310-14
CCGTATC1797050.016.5393455-59
Produced by FastQC (version 0.11.5)