FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences468507855
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG9044710.19305354015889445TruSeq Adapter, Index 13 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2215550.070.5863341
TCGGAAG2379000.065.6296842
CGGAAGA2597100.060.188063
AGAGCAC5272000.030.0520867
AGCACAC5576450.028.395829
GAGCACA5710800.027.8242538
GAAGAGC5790450.027.4628095
TATGCCG1499400.021.17145-49
AAGAGCA8400700.019.2617666
CGTATGC1643950.019.12249245-49
CTCGTAT1529300.019.02865840-44
TCTCGTA1565900.018.51539240-44
ATGCCGT1789900.017.81190945-49
TGCCGTC1810100.017.32637845-49
GGAAGAG9532600.017.0787734
TCGTATG1692950.016.99657640-44
GCCGTCT1854100.016.92460450-54
ATCTCGT1808200.016.14896240-44
CGTCTGA2152250.014.82713515-19
CGTCTTC2159800.014.80563450-54
Produced by FastQC (version 0.11.5)