FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962650

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962650
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451094871
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCTCGTATG10877920.241144838909064TruSeq Adapter, Index 6 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2006300.085.078961
TCGGAAG2159750.078.991132
CGGAAGA2403750.070.982613
AGAGCAC5010450.034.4712457
AGCACAC5375350.032.2202039
GAGCACA5486450.031.526798
GAAGAGC5542450.031.2596935
TATGCCG1585500.021.87140345-49
AAGAGCA8079100.021.7660986
CTCGTAT1664400.020.42039740-44
CGTATGC1718600.020.0644945-49
TCTCGTA1719250.019.78831140-44
GGAAGAG9049700.019.5219084
ATGCCGT1868050.018.69210245-49
TCGTATG1804350.018.57860440-44
TGCCGTC1877500.018.27060145-49
GCCGTCT1931050.017.82889250-54
ATCTCGT1945850.017.56586640-44
CCGGAGA1997200.017.27637130-34
ACTCCGG2051450.017.12551730-34
Produced by FastQC (version 0.11.5)