FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962653

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962653
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences460487833
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC22700610.4929687251910519Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG3446100.0102.688859
GAGCGTC3784600.093.694538
ATCGGAA3924800.091.918251
TCGGAAG4114450.087.794452
AGAGCGT4172850.085.453257
TTCCGAT1457450.084.7140352
CGGAAGA4349800.082.975973
AAGAGCG4602900.077.815626
CTTCCGA1617150.076.5515061
TCCGATC1799350.068.529963
CCGATCT1940750.062.6239624
CGATCTA740600.051.6041875
GAAGAGC7631550.047.3942265
GGAAGAG11434700.032.1057134
CGCCGTA2867750.024.69584355-59
CGATCTG836150.024.6862135
TCGCCGT2966050.023.75129155-59
GTCGCCG3078750.022.98820555-59
GCCGTAT3112050.022.65478355-59
TGGTCGC3259950.022.04937650-54
Produced by FastQC (version 0.11.5)