FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399003856
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG32033100.8028268278189272TruSeq Adapter, Index 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG12265700.042.603392
CGGAAGA12282550.042.5267943
ATCGGAA12335800.042.3559231
AGAGCAC13928500.037.5329177
AGCACAC13905000.037.490579
GAGCACA14135450.036.9639368
GAAGAGC14727350.035.6121565
AAGAGCA16908800.031.1192936
GGAAGAG18038300.029.2779524
TATGCCG6246750.017.07283245-49
GCCGTCT6309750.016.71326850-54
ATGCCGT6383600.016.69888145-49
CGTCTTC6397000.016.6817250-54
CCGTCTT6517500.016.37107350-54
TGCCGTC6341650.016.366745-49
CGTATGC6502300.016.24382445-49
CTCGTAT6432000.015.98549640-44
TCTCGTA6557150.015.67840340-44
TCGTATG6482050.015.42362640-44
ATCTCGT6863600.015.01329640-44
Produced by FastQC (version 0.11.5)