FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498021484
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1843750.044.4580151
TCGGAAG1916150.042.7761732
CGGAAGA2339650.035.120573
AGAGCAC4771050.017.5712597
AGCACAC5085550.016.5387829
GAGCACA5271150.015.9660688
GAAGAGC5672850.014.9534745
CTCGTAT1317850.012.30124740-44
TATGCCG1425250.012.13136345-49
TCTCGTA1386450.011.70305240-44
CGTATGC1473400.011.63158145-49
AAGAGCA8269900.010.5289786
TCGTATG1582150.010.10151740-44
ACACGTC1718750.010.04084910-14
GGAAGAG9120950.09.7235144
ATCTCGT1719200.09.64371240-44
GCCGTCT1811150.09.61512650-54
ATGCCGT1839400.09.59700745-49
CGTCTGA1809300.09.5700715-19
TGCCGTC1970050.08.78242945-49
Produced by FastQC (version 0.11.5)