FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477118751
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGCGATAGATCTCGTATG26714180.5599063114582977TruSeq Adapter, Index 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA4306300.0102.967341
TCGGAAG4470650.099.0517352
CGGAAGA4698600.094.202163
AGAGCAC7348400.060.203827
AGCACAC7655650.057.934389
GAGCACA7782200.057.0099758
GAAGAGC7911550.056.108135
AAGAGCA10551200.042.2931756
GGAAGAG11697850.038.3910374
TATGCCG3561600.025.15533645-49
CTCGTAT3491700.024.25516540-44
CGTATGC3706050.023.93268845-49
TCTCGTA3535450.023.90047540-44
AGCGATA3740550.023.89217830-34
CGATAGA3710300.023.7830635-39
GCGATAG3737650.023.58293730-34
ATGCCGT3840150.023.29630545-49
TGCCGTC3876450.022.7987545-49
GCCGTCT3900050.022.64801450-54
TCGTATG3695700.022.61932240-44
Produced by FastQC (version 0.11.5)