FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962659

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962659
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477118751
Sequences flagged as poor quality0
Sequence length151
%GC40

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGTCCTGGTGTAGATCTC28898230.6056821271314906Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG4326300.0102.85649
GAGCGTC4698250.094.5329368
ATCGGAA4926450.091.243121
TCGGAAG5064200.088.377482
AGAGCGT5038300.088.292787
CGGAAGA5258950.085.014943
AAGAGCG5447350.081.867456
GAAGAGC8495600.052.9189575
GGAAGAG12350450.036.704644
CGCCGTA3564850.024.95161255-59
TCGCCGT3715650.023.86675655-59
GCCGTAT3820200.023.34528255-59
GTCGCCG3871650.022.91633255-59
CCGTATC3942850.022.36534155-59
TGGTCGC4015550.022.31274450-54
GTACGTC4013150.022.16490230-34
CGTATCA4007950.022.07386860-64
TACGTCC4103950.021.84690530-34
GGTCGCC4058700.021.83544750-54
GTGGTCG4174350.021.4847750-54
Produced by FastQC (version 0.11.5)