FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455742250
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1116800.039.833821
ATCGGAA1751450.036.974181
TCGGAAG1921150.033.637112
CGGAAGA2160200.029.955093
TATGCCG1193350.018.4100645-49
CGTATGC1313000.016.66284445-49
CTCGTAT1212300.016.31755440-44
TCTCGTA1248650.015.87388440-44
ATGCCGT1476200.015.00631145-49
GCCGTCT1536000.014.27704350-54
AGAGCAC4737750.014.1170657
ATCTCGT1487100.013.44461140-44
AGCACAC5054300.013.3529769
GAGCACA5165250.013.0619438
GAAGAGC5293150.012.7398675
CGTCTGA1766300.012.53366115-19
ACACGTC1800500.012.3365810-14
CGTCTTC1829200.012.23744950-54
CCGTCTT1856500.012.05437450-54
CACGTCT2277500.09.96985910-14
Produced by FastQC (version 0.11.5)