FastQCFastQC Report
Tue 19 Apr 2022
EGAF00004962661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences455742250
Sequences flagged as poor quality0
Sequence length151
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2075600.031.2906829
GAGCGTC2446750.026.6423518
ATCGGAA2710850.024.5143531
TCGGAAG2861600.023.1146472
AGAGCGT2852050.022.925197
GATCGGA2041600.022.1894281
CGGAAGA3127900.021.1255953
AAGAGCG3370150.019.5899436
CGCCGTA1372300.015.91927955-59
TCGCCGT1514500.014.49439955-59
GCCGTAT1626700.013.56500755-59
GTCGCCG1670500.013.13563355-59
CGTATCA1810250.012.29034760-64
TGGTCGC1825800.012.20462750-54
GTGGTCG1986000.011.32153550-54
GAAGAGC6211950.011.1165165
GCGTCGT1992400.011.04329510-14
CGTCGTG2050200.010.76445410-14
CCGTGTA2239800.010.15052735-39
GAGCCGT2236250.010.02675135-39
Produced by FastQC (version 0.11.5)