FastQCFastQC Report
Fri 3 May 2024
EGAF00004962668

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962668
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2416512935
Total Bases215 Gbp
Sequences flagged as poor quality0
Sequence length89
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAGTTGCTGCAGTTAAAA111647200.4620178041794757Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63436560.2625128095993411No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC43024940.17804556051341805Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAAAGTTGCTGCAGTTAAAAAG31321290.12961358305330156Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTGGTATCAACGCAGAGTACATGGGAAAAGTTGCTGCAGTTAAAAAGCTC24533670.10152509280899008No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)