FastQCFastQC Report
Mon 13 May 2024
EGAF00004962803

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962803
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226162419
Total Bases20.5 Gbp
Sequences flagged as poor quality0
Sequence length91
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA80757233.5707625677633024Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCAAGCACTGCTTATTACAATTTTACTGGGTCTCTATTTTACCCTCCTA33973381.502167342842225No Hit
CAATTTTACTGGGTCTCTATTTTACCCTCCTACAAGCCTCAGAGTACTTC33798311.4944264458013248No Hit
GAAAAATCCACCCCTTACGAGTGCGGCTTCGACCCTATATCCCCCGCCCG33521131.4821706518800544No Hit
CCTAGCAATAATCCCCATCCTCCATATATCCAAACAACAAAGCATAATAT33464391.4796618354174926No Hit
ATTAACCTTTAGCAATAAACGAAAGTTTAACTAAGCTATACTAACCCCAG33315561.4730811665045023No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCTAATTTCCTACTTCCTATT33312151.4729303899070871Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GNNGTATCAACGCAGAGTACATGGGAGNAAAAAAAAAAAAAAAAAAAAAA32999771.4591181923995957No Hit
AGAACAGCTAAAAGAGCACACCCGTCTATGTAGCAAAATAGTGGGAAGAT32553831.4394005044666596No Hit
CTCCAGCACCACGACCCTACTACTATCTCGCACCTGAAACAAGCTAACAT32466461.4355373515880196No Hit
CAAAGCCCATAAAAATAAAAAATTATAACAAACCCTGAGAACCAAAATGA32392571.4322702305372847No Hit
GNTGTTTTTTGTTTTTCAGTTGTTTTGTTTTTTTGTTTTTTTTTTTTTTA32273441.4270027771501683No Hit
GNTCTTTGGACACTAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAAC32099151.4192963685978262No Hit
TTTACGACCTCGATGTTGGATCAGGACATCCCGATGGTGTAGCCGCTATT32061961.4176519751497707No Hit
CTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACATTAC31952001.412789982583269No Hit
GCTCAAAACGCTTAGCCTAGCCACACCCCCACGGGAAACAGCAGTGATTA31881441.4096701008490717No Hit
GGAGGACAACCAGTAAGCTACCCTTTTACCATCATTGGACAAGTAGCATC31779351.4051560882889214No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGCGTAGGAATTATATCCTTCT31621861.3981925087209117Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACTA31455821.3908508822590901No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAATAGGTTTGGTCCTAGCCT31415721.3890778202191054Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT12691100.5611498168491026Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA11317580.5004182414585864Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA5268660.23295912836871452Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA4960680.21934148130950082Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT3532130.15617669883518534Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAA2839130.12553500323146083Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA2423480.10715661827087196Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)