FastQCFastQC Report
Fri 27 May 2022
EGAF00004962823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175453421
Sequences flagged as poor quality0
Sequence length91
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA31743021.8091992632050191Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA31437901.7918088926861107Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA21370481.218014438145381Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA7515350.42833875550366163Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAA6349190.36187325181878327Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAA5875300.33486380410901195Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT5711290.32551602399362733Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA5504900.3137527879835412Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC5097910.2905563180782893Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAA4348720.24785609623422505Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT4010150.22855923681305707Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3936220.22434558286555153No Hit
GTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAA3653980.2082592621548257No Hit
GTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAA3222690.18367780928021915No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAA3085700.1758700390344626Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAA2936520.1673674974966718Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAA2768580.15779572630846567Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA2637440.15032137788866484Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAA2500080.14249251942485636Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAGAAAAAAAAAAAAAAA2327930.13268079851232994Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2247500.12809667586931806Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAA1934590.11026231286764138Clontech SMARTer II A Oligonucleotide (100% over 21bp)
GCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAA1907820.10873655179399438Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCA1826400.10409600391889766Clontech SMARTer II A Oligonucleotide (100% over 23bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)