FastQCFastQC Report
Sat 25 May 2024
EGAF00004962855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144681262
Total Bases13.1 Gbp
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA15396921.064195859723701Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA15005531.0371439806766407Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA8233900.5691061776887183Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA7131510.49291179116200967Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT2765130.19111873657834144Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAA2742710.18956912333264Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAA2700600.18665858748177078Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2577630.17815921456366615Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2433120.16817105175651564No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA2350170.1624377592172233Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT2033170.14052752733107898Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAA1950710.1348281023426517Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAA1908450.1319071988741707Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAA1882140.1300887187450715Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC1765450.12202340341764507Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)