FastQCFastQC Report
Thu 23 May 2024
EGAF00004962863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences188885171
Total Bases17.1 Gbp
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT28737551.5214296520926993Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA20534521.0871430452314332Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA14971980.7926498369742324Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC9651570.5109755280894973Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAATAA9331420.4940260768273863No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT9227400.4885190272559829Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA6275280.3322272450916753Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAATAATC5854340.3099417476240101No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA5483890.29032930276988234Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAAT5361540.28385182233283945Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCACTC3800530.20120848978663336No Hit
GTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTCTAT3430950.18164210466262595No Hit
GTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAA3350220.17736807936076676No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA3266670.17294475700265532Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAA3102840.16427123334102284Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA3019230.15984473444979966No Hit
AGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAATA2906120.15385644011196623Clontech SMARTer II A Oligonucleotide (100% over 21bp)
ATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAATAATCAAC2548300.13491265547786174No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAGATAAAATTTGAAATC2473970.13097746037458918Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAA2452440.12983761440965633No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT2428170.1285527067659536Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCACTCCA2393690.1267272590710681No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2285350.12099149911561875No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT2204860.11673017994620657No Hit
CAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCAC2203340.11664970777404224Clontech SMARTer II A Oligonucleotide (100% over 22bp)
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA2184420.11564804099947051No Hit
GGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTCTATTA2178360.1153272111551838No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCATTATTACCCTCACTGTC2118860.1121771491526987Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATGACCCACCAATCACATGC2108430.11162496181343956Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAA2055570.10882643614198809Clontech SMARTer II A Oligonucleotide (100% over 22bp)
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG2016380.10675163059783024No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCAAAAAAAAAAAAAAAAAA1951060.10329344488350543Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT1947040.1030806171650182No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)