FastQCFastQC Report
Sun 3 Jul 2022
EGAF00004962865

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962865
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185646576
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT29847031.6077339341825512Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA19447831.0475727815200857Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA14134970.7613913654944006Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC9975920.537360839878889Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAATAA9693130.5221281323281718No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT9426170.5077481202777475Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAATAATC6079440.3274738554833352No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA5940260.3199768144390662Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAAT5566810.29986063411156044Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA5146140.2772009110472363Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCACTC3974230.21407504978707498No Hit
GTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTCTAT3522830.18976003090948468No Hit
GCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAA3221240.173514646453808Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAA3176340.17109607235632507No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA3096630.16680243001088263Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAATA3026450.1630221286709861Clontech SMARTer II A Oligonucleotide (100% over 21bp)
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA2999160.16155213118501038No Hit
ATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAATAATCAAC2655680.1430503086682299No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT2500470.13468979896510452Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCACTCCA2495060.1343983850259646No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAGATAAAATTTGAAATC2458910.13245113661563035Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAA2333320.1256861316957443No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2323710.125168481426773No Hit
CAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCAC2300920.12394088000847374Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATGACCCACCAATCACATGC2229580.12009809434890951Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTCTATTA2221470.1196612427691637No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT2148750.11574412231551202No Hit
CTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACCAA2131440.11481170544184989No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTCATTATTACCCTCACTGTC2125210.11447612155260002Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAA1960100.10558234050058646Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTA1951680.10512879052506738Clontech SMARTer II A Oligonucleotide (100% over 24bp)
CCCAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAG1948470.10495588133012483No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGCACATGCAGCGCAAGT1915030.1031546092183246Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGAACT1892660.10194963143300849No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)