FastQCFastQC Report
Thu 16 Jun 2022
EGAF00004962869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197553591
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA67724933.4281801539107435Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA50226072.542402279085881Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA18626300.9428479586584685Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA17303640.8758959992784945Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAA12548810.6352104224721483No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGATAAGGGCAGACGGTGGAT10261960.519451959746963Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAA9965870.504464127913524No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGTAAGGGCGTATGGTGGA9626980.4873097953456083Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA9566090.4842275937165829Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAA8031210.40653323279757536Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAA7738190.3917008018345766Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT7098990.3593450245103365Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAA6564800.33230476686197014Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAA6079240.30772611974438874Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAA5921220.29972727754667844Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA5207130.2635806301288646No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAGAAAAAAAAAAAAAAAA4911880.24863531840329847Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC4710150.2384239120209159Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAA4238800.21456456339485117Clontech SMARTer II A Oligonucleotide (100% over 21bp)
GGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA4211110.21316291841032642No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4025630.20377407363858044No Hit
GTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAAAAAAA3558070.18010657168970418No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAA3538380.17910988011349285Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAA3495060.17691705740747582Clontech SMARTer II A Oligonucleotide (100% over 21bp)
GTGGTATCAACGCAGAGTACATGGGGATAAGGGCAGACGGTGGATGCCTT3170390.16048252952283718No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGACAAAAGGGCGTACGGTGGA3134170.1586491029666983Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAGAAAAAAAAAAAAAAA2979890.1508395764873745Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAA2909390.14727092457661273No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAA2790580.14125686027139844Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTCTAT2734850.1384358535907353No Hit
GTGGTATCAACGCAGAGTACATGGGAAGTAAGGGCGTATGGTGGATGCCT2708250.13708938350809324No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTGAGTAAGGGCACACGGA2595400.13137700949207246Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGAAAAAAAAAAAAAAAAA2445210.1237745154427489Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGGAAAAAAAAAAAAAAAAA2414280.12220886432785724Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAACCCCGTCTCTACTAAA2402330.1216039651741891Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAAAAA2359820.11945214400076382Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAAAA2219060.11232698878148968Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAACCCCATCTCTACTAAA2213180.11202934802637934Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAGGGCGCATGGCGGAT2046510.10359264995593019Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAAAAAAA1975600.10000324418299235No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)