FastQCFastQC Report
Thu 16 Jun 2022
EGAF00004962903

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962903
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205115817
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT41803362.038036881378095Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT34123481.6636201195542126Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC27393571.3355171922212123Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA15136580.7379528415402504Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA11989720.5845341512595297Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAATAA11314980.5516385896266596No Hit
GTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTCTAT10641340.5187966562325128No Hit
GTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCACTC9383490.45747276525242325No Hit
GCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAA8385550.4088202520237627Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTC7712190.37599196945401825Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCA7150670.3486162161741042Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAAT6739240.32855779230326254Clontech SMARTer II A Oligonucleotide (100% over 22bp)
CAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTCT5932780.28924049284799913Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT5839340.28468501773317656Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCAC5694790.27763777963549247Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA4819560.23496774020113717Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTCATTATTACCCTCACTGTC4694960.22889312334211653Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTAGGGAGTCATAAGTGG4421390.21555578037163267Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAATA4146600.20215895880911025Clontech SMARTer II A Oligonucleotide (100% over 21bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA4070510.19844934727778699Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA4009670.19548321814694572Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAATAATC3916080.19092043009047907No Hit
AGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTCTA3729730.18183531892130972Clontech SMARTer II A Oligonucleotide (100% over 21bp)
GGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTCTATTA3701760.18047169906940916No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3618200.17639790304421038No Hit
AGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCACT3380390.16480396536167663Clontech SMARTer II A Oligonucleotide (100% over 21bp)
AGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTA3322020.16195825600324132Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCACTCCA3235010.1577162623202286No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAGATAAAATTTGAAATC3175840.15483155060635817Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATGACCCACCAATCACATGC3055900.1489841224677471Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA2965590.14458124406856446No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTT2832110.13807370106421388Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCC2529200.12330594670814685Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCTGATGTTCGCCGACCGTTG2515180.12262243042914628Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGCACATGCAGCGCAAGT2319990.11310634323241878Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT2283280.11131662264738951Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATGCCCCAACTAAATACTA2167040.10564958040266588Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA2148790.1047598391693021Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGTTGACACAAAATAGACT2132520.10396662876564025Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT2114340.10308030023837703No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)