FastQCFastQC Report
Fri 27 May 2022
EGAF00004962905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205683647
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT42476062.0651160468775625Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT34301201.667667823879066Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC27375961.3309740662076066Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA15708520.763722358540249Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA12404470.6030848918193287Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAATAA11483110.5582898867988275No Hit
GTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTCTAT10662620.5183990149688468No Hit
GTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCACTC9343010.45424175116848253No Hit
GCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAA8532030.414813239868311Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTC7745590.37657782293212644Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCA7138270.34705092524929804Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAAT6848460.3329608405864176Clontech SMARTer II A Oligonucleotide (100% over 22bp)
CAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTCT5972130.2903551199673156Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT5847960.28431817916958657Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCAC5687710.2765270882230127Clontech SMARTer II A Oligonucleotide (100% over 22bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA5010050.24358037564357268Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTCATTATTACCCTCACTGTC4666850.22689455715456078Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCTTTAGGGAGTCATAAGTGG4375460.21272765549514008Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAATA4197870.20409352232071226Clontech SMARTer II A Oligonucleotide (100% over 21bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA4185400.20348725146827057Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAATCTTAGTTCAACTTTAA4022380.19556148768598994Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAATAATC3965380.1927902416082694No Hit
AGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTCTA3733360.18150981152137974Clontech SMARTer II A Oligonucleotide (100% over 21bp)
GGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTCTATTA3700360.17990540589743628No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3587370.17441201827775837No Hit
AGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCACT3370110.16384919506994156Clontech SMARTer II A Oligonucleotide (100% over 21bp)
AGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTA3361800.16344517656282126Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCACTCCA3215970.15635516225555843No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAGATAAAATTTGAAATC3054430.148501353634594Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATGACCCACCAATCACATGC3017740.14671754629088232Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA2945070.14318445063354987No Hit
AGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTT2846610.1383974876719295Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCC2519430.12249053518581378Clontech SMARTer II A Oligonucleotide (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCTGATGTTCGCCGACCGTTG2490420.12108011678731076Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGACAACCTTAGCCAAACCAT2318560.11272456677122221Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATGGCACATGCAGCGCAAGT2283940.11104139941664881Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA2223980.1081262430162958Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGCAGTTGACACAAAATAGACT2149560.10450806524254211Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAATGCCCCAACTAAATACTA2138960.10399271070879057Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAA2106620.10242039319732599No Hit
CAAACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCT2073300.1008004297006655No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)