FastQCFastQC Report
Tue 28 Jun 2022
EGAF00004962921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004962921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201787092
Sequences flagged as poor quality0
Sequence length91
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA35646001.7665153725492018Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA25736321.2754195397196169Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT12350650.612063431688683Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC11256050.5578181383376098Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT11101720.550169978166889Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA10557080.5231791536001719Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA10176650.504326114179791Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAA8529410.4226935387918668No Hit
GTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAA6439110.3191041575642509No Hit
GTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTCTAT5519880.27354970753035085No Hit
GTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCACTC5369990.26612158125555424No Hit
GTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAATAA5170850.25625276368024574No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA5145010.25497220605171317Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAA4259710.21109923126301855Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAA4177830.20704148905619793Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA3950180.1957597961716996No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3898290.1931882739060435No Hit
CAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAA3301630.16361948463978063Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAA3227660.15995373975655489Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAA3065930.15193885642596008No Hit
GGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTCTATTA2971660.14726710071226956No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAA2951130.14624969172953836Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT2906500.1440379546180288Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA2868050.14213248090219766No Hit
GGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCACTCCA2831940.14034297099638068No Hit
GGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAATAATC2734110.13549479170848056No Hit
GTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAAAAAAA2671350.13238458285527996No Hit
AGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAA2636120.13063868327117772Clontech SMARTer II A Oligonucleotide (100% over 21bp)
CAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCAC2408100.11933865422868574Clontech SMARTer II A Oligonucleotide (100% over 22bp)
CAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTTAAT2278890.11293537051418531Clontech SMARTer II A Oligonucleotide (100% over 22bp)
CAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTCT2225830.11030586634352212Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCA2196640.10885929214937098Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAA2104380.10428714637505157No Hit
GCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTC2086930.10342237351832198Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAA2085640.10335844475126289Clontech SMARTer II A Oligonucleotide (100% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2054810.10183059677573429No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)