FastQCFastQC Report
Tue 28 Jun 2022
EGAF00004963019

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963019
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183400228
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA14828400.808526802921968Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA13520660.7372215480560907Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA11639520.6346513375108781Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT11536220.629018847239383Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC10742380.5857342772769072Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT5091350.27760870613530536Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4297080.2343006901823481No Hit
GCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTC4023520.21938467819134883Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCA3862940.21062896388547564Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAGCAAGAGTTTTTGGTG3442900.18772604797416065Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA3038300.16566500669781065No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA2910400.15869118766853443Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAA2813730.153420201854929Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAA2426120.1322855498303961Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAA2365520.12898130093927693Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA2338560.1275112918616437Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2226340.12139243360155473Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAGATAAAATTTGAAATC2174610.11857182642106638Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTCTAT2139410.11665252673513579No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT2117080.11543497099687358Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCACTC2069220.11282537773071907No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)