FastQCFastQC Report
Thu 16 Jun 2022
EGAF00004963021

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963021
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198907606
Sequences flagged as poor quality0
Sequence length91
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAA17177220.8635778362341759Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAA15598790.7842229019638394Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA14284510.7181480028471108Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTT12518140.6293444605632627Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACC11555400.5809430937497685Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAACTTCGCCTTAATTTT5475840.2752956566175755Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTC4353150.21885286779832844Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4260910.21421553884671457No Hit
GCAGTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCA4126010.20743349552957766Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGATAAGCAAGAGTTTTTGGTG3741300.18809235479914227Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAGAAAAAAAAAAAAAAAAAA3349630.1684013028642052Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGGAAAAAAAAAAAAAAAAAAAA3263400.16406612424866246Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAA3263120.16405204736112505No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGAAAAGATAAAATTTGAAATC3082340.15496340547178472Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAA2826090.1420805396451255Clontech SMARTer II A Oligonucleotide (100% over 23bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGAAAAAAAAAAAAAAAAA2761710.13884386100348522Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGAAAAAAAAAAAAAAAAA2667540.13410950207705985Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGAGGCATTGAGGCAGCCAGC2349420.11811614685061364Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGATAGGTTTGGTCCTAGCCTTTCTAT2311720.11622079449289636No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTAGAATCTTAGTTCAACTTT2267940.11401977257722362Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGGCTAAACCTAGCCCCAAACCCACTC2221700.11169507514961494No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)