FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004963714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences394821
Sequences flagged as poor quality0
Sequence length36-90
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGT172734.374893939278812No Hit
GGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGAC153033.8759336509456186No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA88472.2407622694841463No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTG77571.9646877952287238No Hit
GGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGC43021.0896076956392897No Hit
GGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGG23600.5977392286631157No Hit
GGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCG20880.5288472497663498No Hit
GGGCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACT19170.4855364836216919No Hit
GGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG19110.48401680761661614No Hit
GGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG18770.47540531025452043No Hit
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT17760.4498240975024125No Hit
GGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA17660.44729130416061963No Hit
GGGCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTTCTGG16670.42221665007687026No Hit
GGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCCA16520.41841746006418096No Hit
GGGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCC14650.37105422457265447No Hit
GGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAG14190.35940337520040727No Hit
GGGGCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAAC14090.35687058185861437No Hit
GGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCG13570.34370005648129154No Hit
GGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGC13010.3295164137672515No Hit
GGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAA12830.3249573857520243No Hit
GGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG11920.3019089663417093No Hit
GGGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCC10360.2623973902097406No Hit
GGGGCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTTCTG10330.26163755220720275No Hit
GGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTT10060.254799010184362No Hit
GGGAGCCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTTC9620.24365471948047343No Hit
GGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGC9550.24188176414121845No Hit
GGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCA9380.23757601546017057No Hit
GGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGA8530.2160472720549312No Hit
GGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCTGGAGTT7850.19882427733073976No Hit
GGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT7470.18919966263192686No Hit
GGGCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGCACCTC7280.18438735528252043No Hit
GGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACG7120.18033488593565183No Hit
GGGGAGCCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTT7050.17856193059639686No Hit
GGGCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAG7020.17780209259385898No Hit
GGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCTGGTGC6850.17349634391281113No Hit
GGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAGGCCTC6630.16792419856086682No Hit
GGGGCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAA6300.15956598053295037No Hit
GGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCTGGAGT6190.15677990785697823No Hit
GGGTGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT6140.15551351118608178No Hit
GGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATG6140.15551351118608178No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT5780.1463954551556275No Hit
GGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACAGTGC5600.14183642714040032No Hit
GGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCA5350.13550444378591817No Hit
GGGCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGCG5230.13246509177576674No Hit
GGGGCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGCACCT5090.12891918109725675No Hit
GGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACTC4970.1258798290871053No Hit
GGGCTCTTTCCTTGCCTAACGCAGCCATGGCTCGTGGTCCCAAGAAGCAT4680.11853472839590598No Hit
GGGCCTTTCCGTCTGGCGGCAGCCATCAGGTAAGCCAAGATGGGTGCATA4620.11701505239083028No Hit
GGGCTTTTCCTGTGGCAGCAGCCGGGCTGAGAGGAGCGTGGCTGTCTCCT4580.11600193505411313No Hit
GGGCTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAG4560.11549537638575456No Hit
GGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAGGCCT4540.114988817717396No Hit
GGGACAGCAGTCCGTGCCGCCGTCCCGCCCGCCAGCGCCCCAGCGAGGAA4520.1144822590490374No Hit
GGGGCTCTTTCCTTGCCTAACGCAGCCATGGCTCGTGGTCCCAAGAAGCA4500.11397570038067883No Hit
GGGGACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTC4480.11346914171232027No Hit
GGGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCTGGTG4370.11068306903634813No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4110.10409780634768667No Hit
GGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGCC4110.10409780634768667No Hit
GGGCCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGC4070.10308468901096954No Hit
GGGCTTTCCGCTCGGCTGTTTTCCTGCGCAGGAGCCGCAGGGCCGTAGGC4050.10257813034261094No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)