FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21195
Sequences flagged as poor quality0
Sequence length36-90
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGT5422.5572068884170793No Hit
GGGATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCC4862.2929936305732483No Hit
GGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGAC4552.1467327199811277No Hit
GGGATAGTTCTCCCTGAGTGAGACTTACCTGCTCCTCTGGCCCCTGGTCC4081.9249823071479124No Hit
GGGGATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTC3341.5758433592828496No Hit
GGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACTC3171.4956357631516866No Hit
GGGGATAGTTCTCCCTGAGTGAGACTTACCTGCTCCTCTGGCCCCTGGTC3141.4814814814814816No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTG2681.2644491625383345No Hit
GGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCCA2501.179523472517103No Hit
GGGGATTGAGACTTGCCTGCTGCTGTGACCACTGGTCTTGTCCTCTTCTC2471.1653691908468977No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA2221.0474168435951876No Hit
GGGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACT2040.9624911535739562No Hit
GGGGGATTGAGACTTGCCTGCTGCTGTGACCACTGGTCTTGTCCTCTTCT1980.9341825902335457No Hit
GGGATAGTTCTCCCTGATTGAGACTTGCCTGCTGCTGTGACCACTGGTCT1930.9105921207832036No Hit
GGGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCC1820.8586930879924511No Hit
GGGGATAGTTCTCCCTGATTGAGACTTGCCTGCTGCTGTGACCACTGGTC1430.674687426279783No Hit
GGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCCTCCTGCTGC1430.674687426279783No Hit
GGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAAA1210.5708893606982779No Hit
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT1130.5331446095777306No Hit
GGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGC1080.5095541401273885No Hit
GGGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCCTCCTGCTG1010.47652748289690966No Hit
GGGAGATTCTCCCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACCC1010.47652748289690966No Hit
GGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCAG950.4482189195564991No Hit
GGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCTG810.3821656050955414No Hit
GGGGAGATTCTCCCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACC780.36801132342533616No Hit
GGGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA760.3585751356451993No Hit
GGGCTCACAATTGCTCTACAGCTCAGAACAGCAACTGCTGAGGCTGCCTT750.3538570417551309No Hit
GGGGCAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCTC740.34913894786506255No Hit
GGGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAA710.3349846661948573No Hit
GGGGGTCATAGTTCTCCCTGAGTGAGACTTACCTGCTCCTCTGGCCCCTG640.30195800896437835No Hit
GGGAGTTCTCCCTGAGTGAGACTTACCTGCTCCTCTGGCCCCTGGTCCTG630.29723991507431No Hit
GGGGGGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCC620.2925218211842416No Hit
GGGGGAGAGGGGTCATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTG610.28780372729417314No Hit
GGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG580.2736494456239679No Hit
GGGGGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCT580.2736494456239679No Hit
GGGATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTCA570.2689313517338995No Hit
GGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCCAAGCTGTGTTGACT570.2689313517338995No Hit
GGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA560.2642132578438311No Hit
GGGGTCATAGTTCTCCCTGAGTGAGACTTACCTGCTCCTCTGGCCCCTGG550.25949516395376265No Hit
GGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGG540.25477707006369427No Hit
GGGAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCCTG520.2453408822835574No Hit
GGGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCT510.24062278839348905No Hit
GGGCCATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCT510.24062278839348905No Hit
GGGGAGTTCTCCCTGAGTGAGACTTACCTGCTCCTCTGGCCCCTGGTCCT510.24062278839348905No Hit
GGGGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCTCCAGCATGGTGTGTCT500.23590469450342064No Hit
GGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCG490.23118660061335222No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTC480.2264685067232838No Hit
GGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA470.2217504128332154No Hit
GGGGAGTGAGACTTACCTGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCTC460.21703231894314698No Hit
GGGGGAGAGGGGTCATAGTTCTCCCTGAGTGAGACTTACCTGCTCCTCTG440.20759613116301012No Hit
GGGGAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCCT430.2028780372729417No Hit
GGGCAAGGATTACATCGCCCTGAAAGAGGACCTGCGCTCTTGGACCGCGG410.1934418494928049No Hit
GGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTG400.1887237556027365No Hit
GGGCCCTATAATTTGAAATGTGGGTGGAGAGGGGTCATAGTTCTCCCTGA400.1887237556027365No Hit
GGGGTCATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGG400.1887237556027365No Hit
GGGGGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCT400.1887237556027365No Hit
GGGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCCAAGCTGTGTTGAC400.1887237556027365No Hit
GGGAGATCCATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTC380.17928756782259966No Hit
GGGATATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGT370.17456947393253128No Hit
GGGCCTGTTCTCCAGCATGGTGTGTCTGAAGCTCCCTGGAGGCTCCTGCA360.16985138004246286No Hit
GGGAGTTCTAAAGTCCCCACGCACCCACCCGGACTCAGAATCTCCTCAGA350.16513328615239442No Hit
GGGGATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTC340.160415192262326No Hit
GGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG340.160415192262326No Hit
GGGGGTCATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTG330.1556970983722576No Hit
GGGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCC330.1556970983722576No Hit
GGGCCCTTTCATCAGTCTTCCCGCGTCCGCCGATTCCTCCTCCTTGGTCG320.15097900448218918No Hit
GGGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCC320.15097900448218918No Hit
GGGGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCTC320.15097900448218918No Hit
GGGGGGAGAGGGGTCATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCT310.1462609105921208No Hit
GGGGGAGTGAGACTTACCTGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCT310.1462609105921208No Hit
GGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGC310.1462609105921208No Hit
GGGGCTCACAATTGCTCTACAGCTCAGAACAGCAACTGCTGAGGCTGCCT300.14154281670205238No Hit
GGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAG300.14154281670205238No Hit
GGGGCCTGTTCTCCAGCATGGTGTGTCTGAAGCTCCCTGGAGGCTCCTGC290.13682472281198396No Hit
GGGGAGTTCTCCCTGATTGAGACTTGCCTGCTGCTGTGACCACTGGTCTT290.13682472281198396No Hit
GGGGGCAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCT290.13682472281198396No Hit
GGGACACAATTGCTCTACAGCTCAGAACAGCAACTGCTGAGGCTGCCTTG280.13210662892191555No Hit
GGGAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCTCCA280.13210662892191555No Hit
GGGGGGAGAGGGGTCATAGTTCTCCCTGAGTGAGACTTACCTGCTCCTCT270.12738853503184713No Hit
GGGACATCAGATCCATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTC270.12738853503184713No Hit
GGGGCCATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTC270.12738853503184713No Hit
GGGAGAATCTCCTCAGACGCCGAGATGCGGGTCACGGCGCCCCGAACCGT270.12738853503184713No Hit
GGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGC270.12738853503184713No Hit
GGGGGGTCTTGTCCTCTTCTCCAGCATGGTGTGTCTGAAGCTCCCTGGAG270.12738853503184713No Hit
GGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG260.1226704411417787No Hit
GGGCTTTTATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCC250.11795234725171032No Hit
GGGAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACCCTCGTCCTGCTA250.11795234725171032No Hit
GGGGCCCTATAATTTGAAATGTGGGTGGAGAGGGGTCATAGTTCTCCCTG250.11795234725171032No Hit
GGGGATATAGTTCTCCCTGAGTGAGACTTACCTGCTCCTCTGGCCCCTGG250.11795234725171032No Hit
GGGGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTC250.11795234725171032No Hit
GGGGGAAATGTGGGTGGAGAGGGGTCATAGTTCTCCCTGAGTGAGACTTA240.1132342533616419No Hit
GGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTT240.1132342533616419No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGA240.1132342533616419No Hit
GGGACTCCCGAGCTCTACTGACTCCCAAAAGAGCGCCCAAGAAGAAAATG240.1132342533616419No Hit
GGGGATATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGG240.1132342533616419No Hit
GGGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTT240.1132342533616419No Hit
GGGGTTCTCCCTGAGTGAGACTTACCTGCTCCTCTGGCCCCTGGTCCTGT240.1132342533616419No Hit
GGGACTCAGAATCTCCTCAGACGCCGAGATGCGGGTCACGGCGCCCCGAA230.10851615947157349No Hit
GGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCT230.10851615947157349No Hit
GGGGAAATGTGGGTGGAGAGGGGTCATAGTTCTCCCTGAGTGAGACTTAC230.10851615947157349No Hit
GGGATTCTCTTTTCTTTTATTCTTGTCTGTTCTGCCTCACTCCCGAGCTC230.10851615947157349No Hit
GGGCATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTC230.10851615947157349No Hit
GGGGAGTAACTTCTTCCCTATAATTTGAAATGTGGGTGGAGAGGGGTCAT230.10851615947157349No Hit
GGGACTCAGATTCTCCCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGA230.10851615947157349No Hit
GGGAGTTCTCCCTGATTGAGACTTGCCTGCTGCTGTGACCACTGGTCTTG230.10851615947157349No Hit
GGGGGTCTTGTCCTCTTCTCCAGCATGGTGTGTCTGAAGCTCCCTGGAGG230.10851615947157349No Hit
GGGCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGC220.10379806558150506No Hit
GGGGACTCAGAATCTCCTCAGACGCCGAGATGCGGGTCACGGCGCCCCGA220.10379806558150506No Hit
GGGCTTTTCACAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCCT220.10379806558150506No Hit
GGGAGAACAGCAACTGCTGAGGCTGCCTTGGGAAGAGGATGATCCTAAAC220.10379806558150506No Hit
GGGCTGATTGAGACTTGCCTGCTGCTGTGACCACTGGTCTTGTCCTCTTC220.10379806558150506No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)