FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004963718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361084
Sequences flagged as poor quality0
Sequence length36-90
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGT87172.414119706217944No Hit
GGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGAC74962.0759712421486416No Hit
GGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG59081.6361843781502363No Hit
GGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGC47741.3221300306853807No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA40591.124115164338492No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTG36321.0058601322683918No Hit
GGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGG24880.6890363461133697No Hit
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT21240.5882287777913172No Hit
GGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCG21110.584628507494101No Hit
GGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA19510.5403174884514407No Hit
GGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCCA18130.502099234527146No Hit
GGGCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACT17530.4854826023861484No Hit
GGGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCC15290.42344717572642376No Hit
GGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG14860.4115385893587088No Hit
GGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAG14470.4007377784670603No Hit
GGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAA13090.3625195245427657No Hit
GGGGCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAAC12620.3495031626989842No Hit
GGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCG12440.34451817305668486No Hit
GGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCA11810.3270707093086373No Hit
GGGCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTTCTGG11410.3159929545479722No Hit
GGGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCC10980.30408436818025725No Hit
GGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGC10720.29688382758582493No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10410.28829856764630946No Hit
GGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGC9740.2697433284221954No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT9390.2600502930066134No Hit
GGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG9220.25534224723333077No Hit
GGGAGCCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTTC7090.19635320313278903No Hit
GGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCTGGAGTT7040.1949684837877059No Hit
GGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTT7030.1946915399186893No Hit
GGGGCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTTCTG6730.18638322384819045No Hit
GGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGA6700.18555239224114056No Hit
GGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCTGGTGC6630.18361378515802418No Hit
GGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATG6600.1827829535509743No Hit
GGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAGGCCTC6580.18222906581294104No Hit
GGGCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGCACCTC6510.18029045872982463No Hit
GGGCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAG6260.17336686200440896No Hit
GGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCA6160.17059742331424266No Hit
GGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACG5990.16588937754096No Hit
GGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT5730.1586888369465277No Hit
GGGGAGCCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTT5340.1478880260548792No Hit
GGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCTGGAGT5300.1467802505788127No Hit
GGGGCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAA4940.13681027129421408No Hit
GGGCCTTTCCGTCTGGCGGCAGCCATCAGGTAAGCCAAGATGGGTGCATA4810.13321000099699792No Hit
GGGCCTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGCTA4420.12240919010534945No Hit
GGGTGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT4350.12047058302223304No Hit
GGGGCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGCACCT4270.11825503207010003No Hit
GGGCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGCG4170.11548559337993375No Hit
GGGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCTGGTG4000.1107775476066511No Hit
GGGCTCTTTCCTTGCCTAACGCAGCCATGGCTCGTGGTCCCAAGAAGCAT3990.11050060373763446No Hit
GGGCCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGC3930.1088389405235347No Hit
GGGCTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGC3920.10856199665451806No Hit
GGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACTC3910.10828505278550142No Hit
GGGCTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAG3870.10717727730943494No Hit
GGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACAGTGC3780.10468478248828528No Hit
GGGACAGCAGTCCGTGCCGCCGTCCCGCCCGCCAGCGCCCCAGCGAGGAA3730.10330006314320213No Hit
GGGCTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTT3620.10025368058401923No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)