FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963720

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963720
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15750
Sequences flagged as poor quality0
Sequence length36-90
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCC2391.5174603174603174No Hit
GGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCCA2261.434920634920635No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGT2011.2761904761904763No Hit
GGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACTC1911.2126984126984126No Hit
GGGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTC1841.1682539682539683No Hit
GGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCC1761.1174603174603175No Hit
GGGAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTG1581.0031746031746032No Hit
GGGAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTG1530.9714285714285713No Hit
GGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGAC1510.9587301587301587No Hit
GGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCC1510.9587301587301587No Hit
GGGGAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCT1400.8888888888888888No Hit
GGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCAG1390.8825396825396825No Hit
GGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCTG1340.8507936507936508No Hit
GGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCATCCTGCTGC1340.8507936507936508No Hit
GGGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCC1230.780952380952381No Hit
GGGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTC1230.780952380952381No Hit
GGGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACT1190.7555555555555555No Hit
GGGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTC1100.6984126984126984No Hit
GGGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCATCCTGCTG990.6285714285714286No Hit
GGGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA980.6222222222222222No Hit
GGGGAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCT940.5968253968253968No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTG910.5777777777777777No Hit
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT900.5714285714285714No Hit
GGGGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCTC890.5650793650793651No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA870.5523809523809524No Hit
GGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA850.5396825396825398No Hit
GGGGGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCT830.526984126984127No Hit
GGGGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTGT820.5206349206349207No Hit
GGGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCT770.4888888888888889No Hit
GGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTG690.4380952380952381No Hit
GGGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTT660.41904761904761906No Hit
GGGGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTGT650.41269841269841273No Hit
GGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAACA550.3492063492063492No Hit
GGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGC530.33650793650793653No Hit
GGGGATAATTTGGAATGTGGGTGGAGGGGGATCATAGTTCTCCCTGAGTG530.33650793650793653No Hit
GGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCG510.3238095238095238No Hit
GGGATAATTTGGAATGTGGGTGGAGGGGGATCATAGTTCTCCCTGAGTGA500.31746031746031744No Hit
GGGGGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTG490.3111111111111111No Hit
GGGCCATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTTCGTCT490.3111111111111111No Hit
GGGGGGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCC460.2920634920634921No Hit
GGGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCC450.2857142857142857No Hit
GGGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAC410.26031746031746034No Hit
GGGCATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTTCGTCTC400.25396825396825395No Hit
GGGCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGC390.2476190476190476No Hit
GGGCTCACAATTACTCTACAGCTCAGAACACCAACTGCTGAGGCTGCCTT390.2476190476190476No Hit
GGGGAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCT380.24126984126984127No Hit
GGGGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTGTCCTGTTCTC380.24126984126984127No Hit
GGGATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTTCGTCTCA370.23492063492063492No Hit
GGGGGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCT370.23492063492063492No Hit
GGGGCAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCTC370.23492063492063492No Hit
GGGGGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTG370.23492063492063492No Hit
GGGGAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTT350.2222222222222222No Hit
GGGAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCTG350.2222222222222222No Hit
GGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGC350.2222222222222222No Hit
GGGATCATAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGG340.21587301587301586No Hit
GGGGGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTGTCCTGTTCT330.20952380952380953No Hit
GGGAGAGTCTCCTCAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCGT320.20317460317460317No Hit
GGGCCCCATAATTTGGAATGTGGGTGGAGGGGGATCATAGTTCTCCCTGA310.19682539682539685No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTC310.19682539682539685No Hit
GGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGG310.19682539682539685No Hit
GGGAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTTC310.19682539682539685No Hit
GGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG300.19047619047619047No Hit
GGGGCCCCATAATTTGGAATGTGGGTGGAGGGGGATCATAGTTCTCCCTG290.18412698412698414No Hit
GGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCCGAGCTGTGTTGACT280.17777777777777778No Hit
GGGAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCGTCCTCCTGCTGC280.17777777777777778No Hit
GGGGGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTGTCCTGTTCT280.17777777777777778No Hit
GGGGCCATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTTCGTC270.17142857142857143No Hit
GGGGATCATAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTG250.15873015873015872No Hit
GGGCCCATAATTTGGAATGTGGGTGGAGGGGGATCATAGTTCTCCCTGAG240.1523809523809524No Hit
GGGGAGAGTCTCCTCAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCG240.1523809523809524No Hit
GGGCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCTGTGCTCACAGTCA230.14603174603174604No Hit
GGGGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTGTCCTGTTCTC230.14603174603174604No Hit
GGGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCCGAGCTGTGTTGAC230.14603174603174604No Hit
GGGGCTCACAATTACTCTACAGCTCAGAACACCAACTGCTGAGGCTGCCT220.13968253968253969No Hit
GGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCT220.13968253968253969No Hit
GGGGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCTGT210.13333333333333333No Hit
GGGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCC210.13333333333333333No Hit
GGGGCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCTGTGCTCACAGTC210.13333333333333333No Hit
GGGGGAGTCTCCTCAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCGT190.12063492063492064No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG190.12063492063492064No Hit
GGGGGAATGTGGGTGGAGGGGTTCATAGTTCTCCCTGAGTGAGACTTGCC180.1142857142857143No Hit
GGGGCATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTTCGTCT180.1142857142857143No Hit
GGGCCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAG180.1142857142857143No Hit
GGGCTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGC170.10793650793650793No Hit
GGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCG170.10793650793650793No Hit
GGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAA170.10793650793650793No Hit
GGGCAAGGATTACATCGCCCTGAATGAGGACCTGCGCTCCTGGACCGCCG170.10793650793650793No Hit
GGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG170.10793650793650793No Hit
GGGGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTC170.10793650793650793No Hit
GGGGCCCTTTCATCAGTCTTCCCGCGTCCGCCGATTCCTCCTCCTTGGTC170.10793650793650793No Hit
GGGCCGGAGTATTGGGACCGGGAGACACAGATCTCCAAGACCAACACACA160.10158730158730159No Hit
GGGACTCGGCCTCAGTTCCTCATCACTGTTCCTGTGCTCACAGTCATCAA160.10158730158730159No Hit
GGGGAGCTCTACTGACTCCCAACAGAGCGCCCAAGAAGAAAATGGCCATA160.10158730158730159No Hit
GGGGATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTTCGTCTC160.10158730158730159No Hit
GGGAGGGGCGGTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGTGGGT160.10158730158730159No Hit
GGGGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTT160.10158730158730159No Hit
GGGGCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTG160.10158730158730159No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)