FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15750
Sequences flagged as poor quality0
Sequence length36-200
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCACGGCCTCGTCCAGCTCGTAGTTGTGTCTGCACATCCTGTCCGGCAC630.4No Hit
CACGTTCTCCTCCTGGTTATGGAAGTATCTGTCCAGGAACCGCACCCGCT480.3047619047619048No Hit
TTATAGAAGTATCTGTCCAGGAACCGCACCCGCTCCGTCCCATTGAAGAA410.26031746031746034No Hit
GGCTCAACTCGCCGCTGCACTGTGAAGCTCTCCACAACCCCGTAGTTGTG390.2476190476190476No Hit
GGCTCAACTCGCCGCTGCACTGTGAAGCTCTCACCAACCCCGTAGTTGTG320.20317460317460317No Hit
GGCACGGCCTCGTCCAGCTCGTAGTTGTGTCTGCACATCCTGTCCGGCAC240.1523809523809524No Hit
CCGCCTCAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACGTCG240.1523809523809524No Hit
GGTACAGGAAGTAGGCGGTGGTGGCCTGGCCAGCGAGGAGCAGAGTCACC230.14603174603174604No Hit
GATACACAGTCACCTTAGGCTCAACTCGCCGCTGCACTGTGAAGCTCTCA220.13968253968253969No Hit
GCACTGTGAAGCTCTCCACAACCCCGTAGTTGTGTCTGCAGTAGGTGTCC220.13968253968253969No Hit
CTCGTCTTCCAGGATGTCCTTCTGGCTGTTCCAGTACTCGGCATCAGGCC210.13333333333333333No Hit
CTCCACAACCCCGTAGTTGTGTCTGCAGTAGGTGTCCACCGCGGCCCGCG210.13333333333333333No Hit
GGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCA210.13333333333333333No Hit
GTAATTGTCCACCCGGCCCCGCTTCTGCTCCAGGAGGTCCTTCTGGCTGT210.13333333333333333No Hit
GGTCTGGTGTCCCCAGCCAAAGCCAGTGGGGAGCTCAGCACCATCAGTGT200.12698412698412698No Hit
CTCCACAACCCCGTAGTTGTGTCTGCAGTAGGTGTCCACCGCGGCGCGCC190.12063492063492064No Hit
CGGCATCAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACGTCG190.12063492063492064No Hit
CTGTTCATACCCGCGGAGGAAGCGCCCGTCGGGCCCCAGCTCGCAGCCAT180.1142857142857143No Hit
AGGTTGGACTCGCCGCTGCACTGTGAAGCTCTCCACAACCCCGTAGTTGT170.10793650793650793No Hit
GCCTCAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACGTCGCT170.10793650793650793No Hit
CTCACCAACCCCGTAGTTGTGTCTGCAGTAGGTGTCCACCGCGGCGCGCC170.10793650793650793No Hit
GGTTATAGAAGTATCTGTCCAGGAACCGCACCCGCTCCGTCCCATTGAAG170.10793650793650793No Hit
GATACACAGTCACCTTAGGATGGACTCGCCGCTGCACTGTGAAGCTCTCC160.10158730158730159No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)