FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14260
Sequences flagged as poor quality0
Sequence length36-200
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACATCTCATCTTCATCAAATTCAAACATAAACTCCCCTGTTGGTCTAT440.3085553997194951No Hit
GTCCTCATCAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACGT370.2594670406732118No Hit
CTCGTCTTCCAGGATGTCCTTCTGGCTGTTCCAGTACTCGGCATCAGGCC330.23141654978962134No Hit
CACGAACTCCTCCCGGTTGTAGATGTATCTCTCCAGGAAGCGCTGTGTCC320.22440392706872372No Hit
CGCGTACTCCTCCCGGTTGTAGATGTATCTCTCCAGGAAGCGCTGTGTCC320.22440392706872372No Hit
GTAGTTGTCCACCCGGCCCCGCTTCTGCTCCAGGAGGTCCTTCTGGCTGT320.22440392706872372No Hit
ATCTCTCCAGGAACCGCACCCGCTCCGTCCCATTGAAGAAATGACACTCA290.20336605890603085No Hit
GGTTATGGTGCTGCAGGGGCTGGGTCTTTGAAGGATACACAGTCACCTTA280.19635343618513326No Hit
CTCACCAACCCCGTAGTTGTGTCTGCAGTAGGTGTCCACCGCGGCCCGCT260.182328190743338No Hit
GAGACAGCGTGGTGAGTCATATGCGTTTTGGGGGCGTCCGTGCGCTGCAG250.1753155680224404No Hit
GGTACAGGAAGTAGGCGGTGGTGGCCTGGCCAGCGAGGAGCAGAGTCACC250.1753155680224404No Hit
CTCCACAACCCCGTAGTTGTGTCTGCAGTAGTTGTCCACCCGGCCCCGCT240.16830294530154277No Hit
CACGTTCTCCTCCTGGTTATGGAAGTATCTGTCCAGGTACCGCACCCGCT230.16129032258064516No Hit
CACGTTCTCCTCCTGGTTATGGAAGTATCTGTCCAGGAACCGCACCCGCT220.15427769985974754No Hit
GACTTACGCAGCTCCAAGAAACGTGGTCGGGTGTCCCCAGCGAAAGCCAG200.1402524544179523No Hit
GAACTGCTCATCTTCATCAAATTCAAACATAAACTCCCCTGTTGGTCTAT190.1332398316970547No Hit
GACGTAGAGTACTCCAAGAAACGTGGTCTGGTGTCCCCAGCCAAAGCCAG190.1332398316970547No Hit
GCATCAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACGTCGCT190.1332398316970547No Hit
GCACTGTGAAGCTCTCACCAACCCCGTAGTTGTGTCTGCAGTAGGTGTCC190.1332398316970547No Hit
GGCTTGGGAGGCTTGGGAAGCTTCATGCGCAGGTTCTCCAGCTGCAGGTT180.12622720897615708No Hit
GTAATTGTCCACCTGGCCCCGCTTCTGCTCCAGGAGGTCCTTCTGGCTGT180.12622720897615708No Hit
CCTTAGGATGGACTCGCCGCTGCACTGTGAAGCTCTCCACAACCCCGTAG170.11921458625525946No Hit
GTAATTGTCCACCCGGCCCCGCTTCTGCTCCAGGAGGTCCTTCTGGCTGT160.11220196353436186No Hit
GCCACTGGGGTGCTCAGCATCGACAGCATCAAGGTCACAGTTGCTGCCCG160.11220196353436186No Hit
GGTTATGGAAGTATCTGTCCAGGAACCGCACCCGCTCCGTCCCATTGAAG150.10518934081346423No Hit
GTGTCTCCCGGTCCCAATACTCCGGCCCCTCCTGCTCTATCCACGGCGCC150.10518934081346423No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)