FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963727

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963727
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34946
Sequences flagged as poor quality0
Sequence length36-200
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCGTAGCGCGCGTACTCCTCTTGGTTATGGACGCGTCTTTCCAGCAACC760.21747839523836776No Hit
GGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCA760.21747839523836776No Hit
GGGCCCGTTGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA710.20317060607794885No Hit
CCTTAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGAC610.174555027757111No Hit
CTCCTCATCAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACGT570.16310879642877582No Hit
CGGCATCAGGCCGCCCCAGCTCCGTCACCGCCCGGTACTCCCCCACGTCG550.15738568076460827No Hit
GGCGGAGCGCGCGTACTCCTCTTGGTTATGGACGCGTCTTTCCAGCAACC510.1459394494362731No Hit
GGCTCAACTCGCCGCTGCACTGTGAAGCTCTCACCAACCCCGTAGTTGTG490.14021633377210554No Hit
CCGGAGGGTTGCCCATAACAGCATCAGGAGTGGACAGATCCCCAAAGGAC440.1259085446116866No Hit
CTCCACAACCCCGTAGTTGTGTCTGCAGTAGGTGTCCACCGCGGCCCGCG430.12304698677960281No Hit
GACCAGCACGTTGCCCAGGAGCCTGAAGTTCTCAGGATCCACGTGCAGCT420.12018542894751903No Hit
CACCCCGCACGCGCTCCGTCCCGTTGGTGAAGTAGCACAGGCCCTTAAAC420.12018542894751903No Hit
CCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGT400.11446231328335146No Hit
TGGACGCGTCTTTCCAGCAACCGCACCCGCTCCGTCCCGTTGAAGAAATG390.11160075545126767No Hit
CCTGTCTTCCAGGAAGTCCTTCTGGCTGTTCCAGTACTCAGCGTCAGGCC390.11160075545126767No Hit
GAGACAGCGTGGTGAGTCATATGCGTTTTGGGGGCGTCCGTGCGCTGCAG380.10873919761918388No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)