FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004963728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377866
Sequences flagged as poor quality0
Sequence length36-90
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGT84452.2349192570911383No Hit
GGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGAC71991.9051727331911312No Hit
GGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGC50751.3430687068960956No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA40941.0834528642428798No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTG34300.907729195005637No Hit
GGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGG25870.684634235416788No Hit
GGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG23550.6232368088158237No Hit
GGGCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACT21820.5774533829452768No Hit
GGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCCA18890.49991266745354174No Hit
GGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA17470.4623332080684687No Hit
GGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCG17270.45704032646493736No Hit
GGGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCC16690.44169096981469624No Hit
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT16550.4379859526922243No Hit
GGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG16220.42925269804639743No Hit
GGGGCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAAC15300.4049054426701529No Hit
GGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCG14170.3750006616102004No Hit
GGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAG13710.3628270339220782No Hit
GGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG12070.31942540477312065No Hit
GGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTT11710.3098982178867641No Hit
GGGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCC11510.30460533628323266No Hit
GGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAA11030.2919024204347573No Hit
GGGCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTTCTGG10700.2831691657889305No Hit
GGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGC9490.25114723208756545No Hit
GGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGC8720.2307696379139695No Hit
GGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCA8680.22971106159326324No Hit
GGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCTGGAGTT8100.21436170494302212No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7710.20404058581613588No Hit
GGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT7670.2029820094954296No Hit
GGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGA7580.20060021277384046No Hit
GGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCTGGTGC6990.1849862120434228No Hit
GGGCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGCACCTC6720.1778408218786554No Hit
GGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAGGCCTC6490.17175400803459429No Hit
GGGGCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTTCTG6370.16857827907247544No Hit
GGGAGCCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTTC6030.15958038034647204No Hit
GGGCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAG5950.15746322770505947No Hit
GGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCTGGAGT5910.1564046513843532No Hit
GGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACTC5680.15031783754029204No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT5630.1489946171394092No Hit
GGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATG5590.14793604081870293No Hit
GGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACG5590.14793604081870293No Hit
GGGCCTTTCCGTCTGGCGGCAGCCATCAGGTAAGCCAAGATGGGTGCATA5560.1471421085781732No Hit
GGGGCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAA5220.13814420985216982No Hit
GGGCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGCG4970.13152810784775557No Hit
GGGCCTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGCTA4960.13126346376757897No Hit
GGGCCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGC4820.127558446645107No Hit
GGGCTTTTCCTGTGGCAGCAGCCGGGCTGAGAGGAGCGTGGCTGTCTCCT4690.12411807360281157No Hit
GGGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCTGGTG4630.12253020912175215No Hit
GGGCTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGC4570.12094234464069273No Hit
GGGTGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT4530.11988376831998644No Hit
GGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCA4500.11908983607945674No Hit
GGGCTCTTTCCTTGCCTAACGCAGCCATGGCTCGTGGTCCCAAGAAGCAT4500.11908983607945674No Hit
GGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACAGTGC4480.1185605479191036No Hit
GGGATTGCAGACTGCGGAGTCAGACGGCGCTATGTACGCCCTCTTCCTCC4400.11644339527769104No Hit
GGGGAGCCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTT4220.11167980183451277No Hit
GGGGACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTC4220.11167980183451277No Hit
GGGGCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGCACCT4170.1103565814336299No Hit
GGGCTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAG4170.1103565814336299No Hit
GGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCATCCTGCTGC4020.10638692023098135No Hit
GGGCTTTTTCGTCTGGGCTGCCAACATGCCATCCAGACTGAGGAAGACCC3920.10374047942921566No Hit
GGGCTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTT3890.10294654718868593No Hit
GGGCTTTTCACAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCCT3840.10162332678780309No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)