FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22828
Sequences flagged as poor quality0
Sequence length36-90
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCC4832.1158226739092343No Hit
GGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCC4231.852987559137901No Hit
GGGAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTG3391.485018398458034No Hit
GGGGATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTC3281.4368319607499562No Hit
GGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCCA3121.366742596810934No Hit
GGGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTC3101.3579814263185561No Hit
GGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACTC2941.2878920623795338No Hit
GGGGAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCT2391.0469598738391448No Hit
GGGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACT2110.924303486945856No Hit
GGGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCC2000.8761170492377781No Hit
GGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG1790.7841247590678115No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGT1760.7709830033292447No Hit
GGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCAG1740.762221832836867No Hit
GGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGAC1720.7534606623444893No Hit
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT1530.6702295426669003No Hit
GGGGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTGT1500.6570877869283337No Hit
GGGCTCACAATTGCTCTACAGCTCAGAACAGCAACTGCTGAGGCTGCCTT1170.5125284738041003No Hit
GGGGATTGAGACTTGCCTGCTGCTGTGACCACTGGTCTTGTCCTCTTCTC1120.49062554757315574No Hit
GGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCTG1110.4862449623269669No Hit
GGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCATCCTGCTGC1110.4862449623269669No Hit
GGGGGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTG1030.4512002803574558No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA970.4249167688803224No Hit
GGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA950.41615559838794464No Hit
GGGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA910.3986332574031891No Hit
GGGGGATTGAGACTTGCCTGCTGCTGTGACCACTGGTCTTGTCCTCTTCT860.37673033117224464No Hit
GGGATAGTTCTCCCTGATTGAGACTTGCCTGCTGCTGTGACCACTGGTCT850.37234974592605574No Hit
GGGGCAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCTC770.3373050639565446No Hit
GGGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCT740.32416330821797795No Hit
GGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAAA740.32416330821797795No Hit
GGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTG730.31978272297178906No Hit
GGGGATAGTTCTCCCTGATTGAGACTTGCCTGCTGCTGTGACCACTGGTC710.31102155247941127No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTC700.3066409672332224No Hit
GGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGC700.3066409672332224No Hit
GGGATCAGGTCCGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTCA690.3022603819870335No Hit
GGGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCATCCTGCTG680.2978797967408446No Hit
GGGCCATCAGGTCCGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCT660.2891186262484668No Hit
GGGGCTCACAATTGCTCTACAGCTCAGAACAGCAACTGCTGAGGCTGCCT650.2847380410022779No Hit
GGGGAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCCT590.2584545295251445No Hit
GGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAACA550.24093218854038898No Hit
GGGAGAGTCTCCTCAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCGT530.2321710180480112No Hit
GGGCATCAGGTCCGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTC520.22779043280182232No Hit
GGGACACAATTGCTCTACAGCTCAGAACAGCAACTGCTGAGGCTGCCTTG500.21902926230944453No Hit
GGGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCC500.21902926230944453No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTG490.21464867706325563No Hit
GGGATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTCA490.21464867706325563No Hit
GGGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTT490.21464867706325563No Hit
GGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA470.20588750657087787No Hit
GGGGGCAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCT470.20588750657087787No Hit
GGGAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCCTG460.20150692132468898No Hit
GGGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAA450.19712633607850008No Hit
GGGGCCATCAGGTCCGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTC450.19712633607850008No Hit
GGGCCATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCT440.19274575083231119No Hit
GGGGGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCT430.18836516558612232No Hit
GGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGG430.18836516558612232No Hit
GGGCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGC420.18398458033993342No Hit
GGGGTCATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGG420.18398458033993342No Hit
GGGATATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGT400.17522340984755563No Hit
GGGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAC390.17084282460136674No Hit
GGGCGCTTCCTCCGCGGGTACCACCAGTACGCCTACGACGGCAAGGATTA380.16646223935517784No Hit
GGGGGGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCC370.16208165410898898No Hit
GGGCCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAG370.16208165410898898No Hit
GGGCTTTCCTTTCAGCGGAGCGCGGCGGCAAGATGGCAGTGCAAATATCC360.15770106886280008No Hit
GGGAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCTCCA360.15770106886280008No Hit
GGGAGATTCTCCCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACCC350.1533204836166112No Hit
GGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCG340.1489398983704223No Hit
GGGGAGAGTCTCCTCAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCG340.1489398983704223No Hit
GGGCTTTTCACAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCCT330.1445593131242334No Hit
GGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCG330.1445593131242334No Hit
GGGGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTGTCCTGTTCTC330.1445593131242334No Hit
GGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAG330.1445593131242334No Hit
GGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGC330.1445593131242334No Hit
GGGTCTTTTATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTC320.14017872787804453No Hit
GGGGGAGAGGGGTCATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTG320.14017872787804453No Hit
GGGCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGC310.1357981426318556No Hit
GGGATATAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGT310.1357981426318556No Hit
GGGCAAGGATTACATCGCCCTGAAAGAGGACCTGCGCTCTTGGACCGCGG300.1314175573856667No Hit
GGGGAGATTCTCCCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACC300.1314175573856667No Hit
GGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCCAAGCTGTGTTGACT290.12703697213947782No Hit
GGGCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACT290.12703697213947782No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG290.12703697213947782No Hit
GGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCCGAGCTGTGTTGACT280.12265638689328894No Hit
GGGAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTACTTTTCCCTTC280.12265638689328894No Hit
GGGGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCTC280.12265638689328894No Hit
GGGGCTTTCCTTTCAGCGGAGCGCGGCGGCAAGATGGCAGTGCAAATATC280.12265638689328894No Hit
GGGGAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTACTTTTCCCTT280.12265638689328894No Hit
GGGGTCCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGA280.12265638689328894No Hit
GGGAGATCCATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTC280.12265638689328894No Hit
GGGCCTTTTCACAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCC270.11827580164710004No Hit
GGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCT270.11827580164710004No Hit
GGGGGGAGAGGGGTCATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCT270.11827580164710004No Hit
GGGGGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCT270.11827580164710004No Hit
GGGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCCGAGCTGTGTTGAC270.11827580164710004No Hit
GGGCTCTTTCCTTGCCTAACGCAGCCATGGCTCGTGGTCCCAAGAAGCAT270.11827580164710004No Hit
GGGCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCTGTGCTCACAGTCA260.11389521640091116No Hit
GGGAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCGTCCTCCTGCTGC260.11389521640091116No Hit
GGGACTCGGCCTCAGTTCCTCATCACTGTTCCTGTGCTCACAGTCATCAA260.11389521640091116No Hit
GGGGCTCTTTCCTTGCCTAACGCAGCCATGGCTCGTGGTCCCAAGAAGCA260.11389521640091116No Hit
GGGGCTTTTCACAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCC250.10951463115472226No Hit
GGGGCTCACAATTGCTCTACAGCTCAGAGCAGCAACTGCTGAGGCTGCCT250.10951463115472226No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATATC250.10951463115472226No Hit
GGGCCTTTCCTTTCAGCGGAGCGCGGCGGCAAGATGGCAGTGCAAATATC250.10951463115472226No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT250.10951463115472226No Hit
GGGGCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAG250.10951463115472226No Hit
GGGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCC250.10951463115472226No Hit
GGGAGAACAGCAACTGCTGAGGCTGCCTTGGGAAGAGGATGATCCTAAAC250.10951463115472226No Hit
GGGAGTTCTCCCTGATTGAGACTTGCCTGCTGCTGTGACCACTGGTCTTG250.10951463115472226No Hit
GGGCTGGCTAACATTGCTATATTGAACAACAACTTGAATACCTTGATCCA250.10951463115472226No Hit
GGGACAGTTTCAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCAC240.10513404590853338No Hit
GGGGACTCGGCCTCAGTTCCTCATCACTGTTCCTGTGCTCACAGTCATCA240.10513404590853338No Hit
GGGGATATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGG240.10513404590853338No Hit
GGGGCCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAA240.10513404590853338No Hit
GGGCTCACAATTGCTCTACAGCTCAGAGCAGCAACTGCTGAGGCTGCCTT230.10075346066234449No Hit
GGGGATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTC230.10075346066234449No Hit
GGGCCCTTTCATCAGTCTTCCCGCGTCCGCCGATTCCTCCTCCTTGGTCG230.10075346066234449No Hit
GGGGCCCTTTCATCAGTCTTCCCGCGTCCGCCGATTCCTCCTCCTTGGTC230.10075346066234449No Hit
GGGCGCCTCCTCCGCGGGCATAACCAGTTCGCCTACGACGGCAAGGATTA230.10075346066234449No Hit
GGGAGTAACTTCTTCCCTATAATTTGGAATGTGGGTGGAGAGGGGTCATA230.10075346066234449No Hit
GGGCCCTTTCTTTGCGGAATCACCATGGCGGCTGGGACCCTGTACACGTA230.10075346066234449No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)