FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22828
Sequences flagged as poor quality0
Sequence length36-200
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTCATCAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACGT640.28035745575608906No Hit
GCATCAGGCCGCCCCAGCTCCGTCACCGCCCGGTACTCCCCCACGTCGCT630.27597687050990016No Hit
GCAGCAGGCCGCCCCAGCTCCGTCACCGCCCGGTACTCCCCCACGTCGCT550.24093218854038898No Hit
GCGCTAGGCCGCCCCAGCTCCGTCACCGCCCGGTACTCCCCCACGTCGCT550.24093218854038898No Hit
GCACCGTGAAGCTCTCACCAACCCCGTAGTTGTGTCTGCAGTAGGTGTCC450.19712633607850008No Hit
GGTCTGGTGTCCCCAGCCAAAGCCAGTGGGGAGCTCAGCACCATCAGTGT390.17084282460136674No Hit
GCGCGAGGCCGCCCCAGCTCCGTCACCGCCCGGTACTCCCCCACGTCGCT380.16646223935517784No Hit
CTGTTCATACCCGCGGAGGAAGCGCCTGTCGGGCCCCAGCTCGCAGCCAT330.1445593131242334No Hit
CACCCGTAGAGTACTCCAAGAAACGTGGTCTGGTGTCCCCAGCCAAAGCC320.14017872787804453No Hit
TGGTTATAGAAGTATCTGTCCAGGAACCGCACCCGCTCCGTCCCATTGAA280.12265638689328894No Hit
GCAGCTGGCCGCCCCAGCTCCGTCACCGCCCGGTACTCCCCCACGTCGCT260.11389521640091116No Hit
CGCCGCATCAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACGT230.10075346066234449No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)