FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13994
Sequences flagged as poor quality0
Sequence length36-200
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACGTTCTCCTCCTGGTTATGGAAGTATCTGTCCAGGAACCGCACCCGCT640.45733885951121905No Hit
CTCCACAACCCCGTAGTTGTGTCTGCAGTAGGTGTCCACCGCGGCCCGCT450.32156638559382594No Hit
GGCTCAACTCGCCGCTGCACTGTGAAGCTCTCACCAACCCCGTAGTTGTG410.2929827068743747No Hit
GGCTCAACTCGCCGCTGCACTGTGAAGCTCTCCACAACCCCGTAGTTGTG340.24296126911533514No Hit
CCACCGCGGCCCGCCTCTGCTCCAGGAGGTCCTTCTGGCTGTTCCAGTAC280.20008575103615836No Hit
CTCGTCTTCCAGGATGTCCTTCTGGCTGTTCCAGTACTCGGCATCAGGCC270.19293983135629555No Hit
GGTCTGGTGTCCCCAGCCAAAGCCAGTGGGGAGCTCAGCACCATCAGTGT250.17864799199656997No Hit
GGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCA220.15721023295698155No Hit
GTAATTGTCCACCTGGCCCCGCTTCTGCTCCAGGAGGTCCTTCTGGCTGT210.15006431327711875No Hit
CTCCACAACCCCGTAGTTGTGTCTGCAGTAGGTGTCCACCGCGGCCCGCC210.15006431327711875No Hit
GCACTGTGAAGCTCTCCACAACCCCGTAGTTGTGTCTGCAGTAGGTGTCC200.14291839359725597No Hit
GAGACGGGATGGTGGGTCACGTGTGTCTTTGGGTGTTCCGCGCGCTGCAG190.13577247391739317No Hit
TGGAAGTATCTGTCCAGGAACCGCACCCGCTCCGTCCCATTGAAGAAATG180.12862655423753036No Hit
CGCGGCTTCCAGGATGTCCTTCTGGCTGTTCCAGTACTCGGCATCAGGCC180.12862655423753036No Hit
CTCACCAACCCCGTAGTTGTGTCTGCAGTAGGTGTCCACCGCGGCCCGCC170.12148063455766757No Hit
GTGTCTCCCGGTCCCAATACTCCGGCCCCTCCTGCTCTATCCACGGCGCC160.11433471487780476No Hit
CCTCCTGGTTATGGAAGTATCTGTCCAGGAACCGCACCCGCTCCGTCCCA160.11433471487780476No Hit
CACGTTCTCCTCCTGGTTATAGATGCATCTTTCCAGCAACCGCACCCGCT160.11433471487780476No Hit
CTCACCAACCCCGTAGTTGTGTCTGCAGTAGGTGTCCACCGCGGCCCGCT150.10718879519794197No Hit
TTCCAGCAACCGCACCCGCTCCGTCCCATTGAAGAAATGACACTCAGACG150.10718879519794197No Hit
ACTCTGACCCCTCCTGCTCCATCCACGGCGCCCGCGGCACCATCCTCGGA140.10004287551807918No Hit
CCTCTTGGTTATAGATGCATCTTTCCAGCAACCGCACCCGCTCCGTCCCA140.10004287551807918No Hit
CACATGCCCTTAAACTGGTACACGAAATCCTCGGGAGAGTCTCTGCCCTC140.10004287551807918No Hit
CTCGTCTTCCAGGAGGTCCTTCTGGCTGTTCCAGTACTCGGCATCAGGCC140.10004287551807918No Hit
CACCCTGTCCACCGACGCCCGGGCCCCCTCCAGGACTTCCTTCTGGCTGT140.10004287551807918No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)