FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004963744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences735106
Sequences flagged as poor quality0
Sequence length36-90
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG150912.0529012142466527No Hit
GGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGAC128691.750631881660604No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGT126571.7217925033940682No Hit
GGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGC81191.1044665667264313No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA68740.935103236812106No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTG50410.6857514426490874No Hit
GGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGG42840.5827730966690519No Hit
GGGCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACT42740.5814127486376114No Hit
GGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA38040.5174763911599143No Hit
GGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCG37760.5136674166718813No Hit
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT37540.5106746510027125No Hit
GGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG31750.43191049998231545No Hit
GGGGCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAAC30460.41436201037673476No Hit
GGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAG30250.41150527951071003No Hit
GGGCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTTCTGG29180.3969495555742981No Hit
GGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCCA27480.373823639039812No Hit
GGGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCC27380.37246329100837156No Hit
GGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCA26430.3595399847096881No Hit
GGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAA25380.3452563303795643No Hit
GGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCG23660.32185834423879No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT20860.2837685993584599No Hit
GGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGC20130.27383805872894523No Hit
GGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGC19180.26091475243026174No Hit
GGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG18480.2513923162101792No Hit
GGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGA18040.24540678487184162No Hit
GGGGCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTTCTG17960.24431850644668932No Hit
GGGCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAG16200.2203763810933389No Hit
GGGAGCCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTTC16200.2203763810933389No Hit
GGGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCC16050.2183358590461784No Hit
GGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCTGGTGC15790.21479895416443343No Hit
GGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTT15450.2101737708575362No Hit
GGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCTGGAGTT14550.19793063857457294No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14540.19779460377142888No Hit
GGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACG14430.19629822093684446No Hit
GGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAGGCCTC14220.19344149007081973No Hit
GGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATG14060.19126493322051513No Hit
GGGCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGCACCTC13090.1780695573155436No Hit
GGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCTGGAGT12870.1750767916463748No Hit
GGGGCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAA12780.17385247841807847No Hit
GGGCCTTTCCGTCTGGCGGCAGCCATCAGGTAAGCCAAGATGGGTGCATA12410.16881919070174914No Hit
GGGCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGCG12140.16514625101686017No Hit
GGGCTCTTTCCTTGCCTAACGCAGCCATGGCTCGTGGTCCCAAGAAGCAT12060.16405797259170787No Hit
GGGTGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT11790.16038503290681888No Hit
GGGGAGCCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTT11560.15725623243450604No Hit
GGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCA11490.1563039888124978No Hit
GGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT11410.1552157103873455No Hit
GGGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCTGGTG10590.14406085652953451No Hit
GGGCCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGC10510.14297257810438221No Hit
GGGCTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAG10400.14147619526979782No Hit
GGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACTC10250.13943567322263728No Hit
GGGCTCTACCGGCGGGATTTGATGGCGTGATGTCTCACAGAAAGTTCTCC9180.1248799492862254No Hit
GGGCTTTCCAACTTGGACGCTGCAGAATGGCTCCCGCAAAGAAGGGTGGC9070.123383566451641No Hit
GGGGCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGCACCT8980.12215925322334466No Hit
GGGGCTCTTTCCTTGCCTAACGCAGCCATGGCTCGTGGTCCCAAGAAGCA8920.12134304440448043No Hit
GGGCTTTTCCTGTGGCAGCAGCCGGGCTGAGAGGAGCGTGGCTGTCTCCT8830.12011873117618412No Hit
GGGCTTTTTCGTCTGGGCTGCCAACATGCCATCCAGACTGAGGAAGACCC8720.11862234834159971No Hit
GGGGCCTTTCCGTCTGGCGGCAGCCATCAGGTAAGCCAAGATGGGTGCAT8660.1178061395227355No Hit
GGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAGGCCT8510.11576561747557496No Hit
GGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACAGTGC8490.11549354786928687No Hit
GGGCCTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGCTA8470.1152214782629988No Hit
GGGCCTTTCCGCTCGGCTGTTTTCCTGCGCAGGAGCCGCAGGGCCGTAGG8370.11386113023155843No Hit
GGGCCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAA8160.11100439936553369No Hit
GGGATGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT8020.10909991212151716No Hit
GGGCTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTT7970.108419738105797No Hit
GGGCTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGC7910.10760352928693277No Hit
GGGCTCTTTCTCGACTCCATCTTCGCGGTAGCTGGGACCGCCGTTCAGTC7680.10447472881461993No Hit
GGGGCTTTCCAACTTGGACGCTGCAGAATGGCTCCCGCAAAGAAGGGTGG7640.1039305896020438No Hit
GGGGCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGC7620.1036585199957557No Hit
GGGCTTTTCACAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCCT7530.10243420676745939No Hit
GGGCTCTTTCCCATCTTGCAAGATGGCGGGTGAAAAAGTTGAGAAGCCAG7490.10189006755488325No Hit
GGGGCTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGA7360.10012161511401077No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)