FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963748

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963748
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35337
Sequences flagged as poor quality0
Sequence length36-90
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCC8652.447859184424258No Hit
GGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCC7162.0262048278008886No Hit
GGGGATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTC5581.5790814160794635No Hit
GGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCAG4841.3696691852732263No Hit
GGGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTC4581.296091914989954No Hit
GGGAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTG4391.242323909782947No Hit
GGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCCA3741.0583807340747657No Hit
GGGGAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCT3390.9593344086934374No Hit
GGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACTC3200.9055664034864307No Hit
GGGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA3120.8829272433992699No Hit
GGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA3040.8602880833121092No Hit
GGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG2960.8376489232249484No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGT2580.7301129128109347No Hit
GGGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCC2550.7216232277782495No Hit
GGGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACT2290.648045957494977No Hit
GGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCATCCTGCTGC2240.6338964824405015No Hit
GGGGGATTGAGACTTGCCTGCTGCTGTGACCACTGGTCTTGTCCTCTTCT2190.619747007386026No Hit
GGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGAC2140.6055975323315506No Hit
GGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCTG2020.5716387922008094No Hit
GGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGC1980.5603192121572289No Hit
GGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTG1870.5291903670373829No Hit
GGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCG1840.5207006820046977No Hit
GGGATAGTTCTCCCTGATTGAGACTTGCCTGCTGCTGTGACCACTGGTCT1750.49523162690664174No Hit
GGGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCC1720.48674194187395653No Hit
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT1650.46693267679769085No Hit
GGGGATTGAGACTTGCCTGCTGCTGTGACCACTGGTCTTGTCCTCTTCTC1580.44712341172142517No Hit
GGGGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTGT1550.4386337266887398No Hit
GGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCC1530.43297393666694967No Hit
GGGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCATCCTGCTG1440.40750488156889375No Hit
GGGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCT1400.39618530152531345No Hit
GGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG1320.3735461414381526No Hit
GGGGGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTG1300.36788635141636244No Hit
GGGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTT1270.3593966663836772No Hit
GGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGG1260.35656677137278203No Hit
GGGAGATTCTCCCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACCC1200.3395874013074115No Hit
GGGGATAGTTCTCCCTGATTGAGACTTGCCTGCTGCTGTGACCACTGGTC1160.32826782126383114No Hit
GGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAAA1140.3226080312420409No Hit
GGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAACA1090.30845855618756546No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTG1070.3027987661657753No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA1060.29996887115488013No Hit
GGGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTC1010.2858193961004047No Hit
GGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGC990.28015960607861445No Hit
GGGCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGC950.2688400260350341No Hit
GGGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCC930.2631802360132439No Hit
GGGAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTTTTCCCTTCGTC910.25752044599145374No Hit
GGGAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCCTG890.25186065596966356No Hit
GGGGAGATTCTCCCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACC870.24620086594787333No Hit
GGGGCAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCTC860.24337097093697826No Hit
GGGGAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCCT810.22922149588250276No Hit
GGGAGAGTCTCCTCAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCGT810.22922149588250276No Hit
GGGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAC810.22922149588250276No Hit
GGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA750.21224212581713217No Hit
GGGGTCATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGG700.19809265076265672No Hit
GGGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAA700.19809265076265672No Hit
GGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAG650.18394317570818122No Hit
GGGCCATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCT640.18111328069728613No Hit
GGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTT620.17545349067549593No Hit
GGGATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTCA610.17262359566460084No Hit
GGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAA600.16979370065370575No Hit
GGGGAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTTTTCCCTTCGT600.16979370065370575No Hit
GGGGCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTG600.16979370065370575No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTC580.16413391063191557No Hit
GGGGGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTGTCCTGTTCT580.16413391063191557No Hit
GGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCG580.16413391063191557No Hit
GGGTCTTTTATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTC570.16130401562102045No Hit
GGGGAGAGTCTCCTCAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCG570.16130401562102045No Hit
GGGCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCTGTGCTCACAGTCA550.15564422559923027No Hit
GGGGGTCATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTG540.15281433058833516No Hit
GGGAGATCCATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTC530.14998443557744007No Hit
GGGATATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGT530.14998443557744007No Hit
GGGCCCTTTCATCAGTCTTCCCGCGTCCGCCGATTCCTCCTCCTTGGTCG520.14715454056654498No Hit
GGGCGCCTCCTCCGCGGGCATAACCAGTACGCCTACGACGGCAAGGATTA500.1414947505447548No Hit
GGGGGAGAGGGGTCATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTG500.1414947505447548No Hit
GGGGGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCT500.1414947505447548No Hit
GGGGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTT500.1414947505447548No Hit
GGGGGGAGAGGGGTCATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCT480.1358349605229646No Hit
GGGCTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGC470.1330050655120695No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCCTACTACCAGACAACCTT470.1330050655120695No Hit
GGGCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACT470.1330050655120695No Hit
GGGATTCTCTTTTCTTTTATTCTTGTCTGTTCTGCCTCACTCCCGAGCTC460.13017517050117441No Hit
GGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGC460.13017517050117441No Hit
GGGGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTC450.1273452754902793No Hit
GGGATGTCTCTTGTCAGCTGTCTTTCAGAAGACCTGGTGGGGCAAGTCCG450.1273452754902793No Hit
GGGACTCGGCCTCAGTTCCTCATCACTGTTCCTGTGCTCACAGTCATCAA450.1273452754902793No Hit
GGGGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTGTCCTGTTCTC440.12451538047938422No Hit
GGGAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCC440.12451538047938422No Hit
GGGGCCCTTTCATCAGTCTTCCCGCGTCCGCCGATTCCTCCTCCTTGGTC440.12451538047938422No Hit
GGGCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCC420.11885559045759402No Hit
GGGACAGTTTCAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCAC410.11602569544669893No Hit
GGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCCAAGCTGTGTTGACT410.11602569544669893No Hit
GGGCTCACAATTGCTCTACAGCTCAGAACAGCAACTGCTGAGGCTGCCTT400.11319580043580384No Hit
GGGGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCTC390.11036590542490873No Hit
GGGGATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTC380.10753601041401364No Hit
GGGAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCGTCCTCCTGCTGC380.10753601041401364No Hit
GGGTCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTT370.10470611540311855No Hit
GGGGACTTGGGTTCATGGTCTCTAATATTTCAAACAGGAGCTCCCTTTAG370.10470611540311855No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGA370.10470611540311855No Hit
GGGTGGACAACTACTGCAGACACAACTACGGGGTTGTGGAGAGCTTCACA370.10470611540311855No Hit
GGGCCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAG370.10470611540311855No Hit
GGGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCCAAGCTGTGTTGAC370.10470611540311855No Hit
GGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCTGGTGC360.10187622039222344No Hit
GGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG360.10187622039222344No Hit
GGGGGCAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCT360.10187622039222344No Hit
GGGGACTCAGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGA360.10187622039222344No Hit
GGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGA360.10187622039222344No Hit
GGGACTTGGGTTCATGGTCTCTAATATTTCAAACAGGAGCTCCCTTTAGC360.10187622039222344No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)