FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35337
Sequences flagged as poor quality0
Sequence length36-200
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTCATCAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACGT2110.5971078472988652No Hit
CCTCCTGGTTATGGAAGTATCTGTCCAGGTACCGCACCCGCTCCGTCCCA1350.3820358264708379No Hit
GGATAGACTCGCCGCTGCACTGTGAAGCTCTCCACAACCCCGTAGTTGTG1190.3367575062965164No Hit
GGTACACGTAATTCTCTGGAGTGGCCCTGCCCTGGACCACAGATGTGAGC1160.32826782126383114No Hit
CCTCAGGATAGACTCGCCGCTGCACTGTGAAGCTCTCCACAACCCCGTAG1120.3169482412202507No Hit
CGCGTACTCCTCCCGGTTGTAGATGTATCTCTCCAGGAAGCGCTGTGTCC770.2179019158389224No Hit
GTCACGGCCTCGTCCAGCTCGTAGTTGTGTCTGCATACCCTGTCCGGCAC760.2150720208280273No Hit
GAACATCTCATCTTCATCAAATTCAAACATAAACTCCCCTGTTGGTCTAT720.20375244078444688No Hit
GGTCTGGTGTCCCCAGCCAAAGCCAGTGGGGAGCTCAGCACCATCAGTGT690.1952627557517616No Hit
GCACATCCTGTCCGGCACTGCCCGCTCCTCCTCCAGGATGTCCTTCTGGC660.1867730707190763No Hit
GTAGTTGTCCACCCGGCCCCGCTTCTGCTCCAGGAGGTCCTTCTGGCTGT650.18394317570818122No Hit
CACGAACTCCTCCCGGTTGTAGATGTATCTCTCCAGGAAGCGCTGTGTCC560.15847412061012536No Hit
CCTCCTGGTTATGGAAGTATCTGTCCAGGAACCGCACCCGCTCCGTCCCG560.15847412061012536No Hit
GGTTATGGTGCTGCAGGGGCTGGGTCTTTGAAGGATACACAGTCACCTTA560.15847412061012536No Hit
GGTACAGGAAGTAGGCGGTGGTGGCCTGGCCAGCGAGGAGCAGAGTCACC550.15564422559923027No Hit
GCATACCCTGTCCGGCACTGCCCGCTTCTCCTCCAGGATGTCCTTCTGGC490.1386648555338597No Hit
GCACTGTGAAGCTCTCCACAACCCCGTAGTTGTGTCTGCAGTAGGTGTCC490.1386648555338597No Hit
TGTTTAACCTGCTCCAAGAAACGTGGTCGGGTGTCCCCAGCCAAAGCCAG460.13017517050117441No Hit
CACACTTAGCCTGCTCCAAGAAACGTGGTCGGGTGTCCCCAGCCAAAGCC440.12451538047938422No Hit
GTCACGGCCTCGTCCAGCTCGTAGTTGTGTCTGCACATCCTGTCCGGCAC440.12451538047938422No Hit
GAACTGCTCATCTTCATCAAATTCAAACATAAACTCCCCTGTTGGTCTAT430.12168548546848913No Hit
GCAGTAGGTGTCCACCTCGGCCCGCCTCTGCTCCAGGAGGTCCTTCTGGC420.11885559045759402No Hit
CACACTTAGCCTGCTCCAAGAAACGTGGTTGGGTGTCCCCAGCCAAAGCC410.11602569544669893No Hit
GGTCATACCCGCGGAGGAGGCGCCCGTCGGGCCCCACGTCGCAGCCATAC390.11036590542490873No Hit
CCGCCTGGTTATGGAAGTATCTGTCCAGGTACCGCACCCGCTCCGTCCCA380.10753601041401364No Hit
GCACATCCTGTCCGGCACTGCCCGCTTCTCCTCCAGGATGTCCTTCTGGC370.10470611540311855No Hit
CACGTTCTCCTCCTGGTTATGGAAGTATCTGTCCAGGTACCGCACCCGCT370.10470611540311855No Hit
GTTATGGAAGTATCTGTCCAGGTACCGCACCCGCTCCGTCCCATTGAAGA370.10470611540311855No Hit
ACCCCGTAGTTGTGTCTGCAGTAGGTGTCCACCTCGGCCCGCCTCTGCTC360.10187622039222344No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)