FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30097
Sequences flagged as poor quality0
Sequence length36-90
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCC7112.362361697179121No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGT5761.9138120078413132No Hit
GGGGATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTC4551.5117785825829817No Hit
GGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCC4351.4453267767551583No Hit
GGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGAC4291.4253912350068114No Hit
GGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACTC3981.322390935973685No Hit
GGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCCA3761.2492939495630793No Hit
GGGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTC3081.02335780974848No Hit
GGGAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTG2910.96687377479483No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTG2780.9236801010067448No Hit
GGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG2650.8804864272186597No Hit
GGGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACT2570.8539057048875304No Hit
GGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCAG2530.8406153437219656No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA2470.8206798019736186No Hit
GGGGAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCT2080.691098780609363No Hit
GGGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCC2040.6778084194437984No Hit
GGGGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTGT1720.5714855301192809No Hit
GGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGC1560.5183240854570224No Hit
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT1540.51167890487424No Hit
GGGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA1420.47180782137754596No Hit
GGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA1360.4518722796291989No Hit
GGGGATTGAGACTTGCCTGCTGCTGTGACCACTGGTCTTGTCCTCTTCTC1350.44854968933780776No Hit
GGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCTG1270.42196896700667835No Hit
GGGGCAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCTC1200.39871083496694026No Hit
GGGATAGTTCTCCCTGATTGAGACTTGCCTGCTGCTGTGACCACTGGTCT1200.39871083496694026No Hit
GGGAGATTCTCCCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACCC1190.39538824467554906No Hit
GGGGGATTGAGACTTGCCTGCTGCTGTGACCACTGGTCTTGTCCTCTTCT1150.3820978835099844No Hit
GGGGAGATTCTCCCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACC1130.375452702927202No Hit
GGGATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTCA1080.3588397514702462No Hit
GGGCCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCT1010.33558161943050807No Hit
GGGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCC1000.33225902913911687No Hit
GGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAAA940.3123234873907698No Hit
GGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGG930.30900089709937867No Hit
GGGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCT900.29903312622520517No Hit
GGGAGAGTCTCCTCAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCGT880.2923879456424228No Hit
GGGAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCCTG840.2790975844768582No Hit
GGGGATAGTTCTCCCTGATTGAGACTTGCCTGCTGCTGTGACCACTGGTC830.275774994185467No Hit
GGGCCATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCT820.2724524038940758No Hit
GGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAACA820.2724524038940758No Hit
GGGATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTCA790.2624846330199023No Hit
GGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCCAAGCTGTGTTGACT780.2591620427285112No Hit
GGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTG760.25251686214572877No Hit
GGGGAGAGTCTCCTCAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCG760.25251686214572877No Hit
GGGGGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTG750.24919427185433762No Hit
GGGAGATCCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTC750.24919427185433762No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTC730.2425490912715553No Hit
GGGAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCGTCCTCCTGCTGC710.23590391068877298No Hit
GGGCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTC700.23258132039738177No Hit
GGGGGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTGTCCTGTTCT680.22593613981459945No Hit
GGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG660.21929095923181713No Hit
GGGCTCACAATTGCTCTACAGCTCAGAACAGCAACTGCTGAGGCTGCCTT620.20600059806625245No Hit
GGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCG610.20267800777486128No Hit
GGGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTT600.19935541748347013No Hit
GGGGAGATCCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTT600.19935541748347013No Hit
GGGGTCATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGG580.19271023690068778No Hit
GGGCATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTC560.18606505631790543No Hit
GGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCTGAGCTGTGTTGACT550.18274246602651428No Hit
GGGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAA540.1794198757351231No Hit
GGGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCC540.1794198757351231No Hit
GGGAGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCT530.17609728544373193No Hit
GGGGAGTCTCCTCAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCGTC520.17277469515234076No Hit
GGGGGCAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCT520.17277469515234076No Hit
GGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCATCCTGCTGC490.16280692427816726No Hit
GGGGCCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTC490.16280692427816726No Hit
GGGGAGATCCATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTT480.15948433398677608No Hit
GGGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCCAAGCTGTGTTGAC480.15948433398677608No Hit
GGGTCTTTTATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTC460.15283915340399376No Hit
GGGGAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCCT460.15283915340399376No Hit
GGGGGAGAGGGGTCATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTG460.15283915340399376No Hit
GGGAGATCCATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTC460.15283915340399376No Hit
GGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTT450.14951656311260259No Hit
GGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCG450.14951656311260259No Hit
GGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG450.14951656311260259No Hit
GGGGGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCT440.1461939728212114No Hit
GGGCAAGGATTACATCGCCCTGAAAGAGGACCTGCGCTCTTGGACCGCGG440.1461939728212114No Hit
GGGGGGAGAGGGGTCATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCT440.1461939728212114No Hit
GGGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAC440.1461939728212114No Hit
GGGGAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCGTCCTCCTGCTG440.1461939728212114No Hit
GGGGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTGTCCTGTTCTC430.14287138252982023No Hit
GGGAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCTCCA430.14287138252982023No Hit
GGGGATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTC420.1395487922384291No Hit
GGGGATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTC410.1362262019470379No Hit
GGGATATAGTTCTCCCTGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGT410.1362262019470379No Hit
GGGGGAGTCTCCTCAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCGT400.13290361165564674No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT380.12625843107286439No Hit
GGGGAGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCC380.12625843107286439No Hit
GGGGCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCT370.12293584078147324No Hit
GGGCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGC370.12293584078147324No Hit
GGGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCTGAGCTGTGTTGAC370.12293584078147324No Hit
GGGGCCATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTC360.11961325049008208No Hit
GGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA360.11961325049008208No Hit
GGGATCCACAGGTTTTTATTCTTTCTGCCAGGTACATCAGATCCATCAGG350.11629066019869089No Hit
GGGAGAATCTCCTCAGACGCCGAGATGCGGGTCACGGCGCCCCGAACCCT350.11629066019869089No Hit
GGGGAGTGAGACTCACCTGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCTC350.11629066019869089No Hit
GGGGCTCACAATTGCTCTACAGCTCAGAACAGCAACTGCTGAGGCTGCCT350.11629066019869089No Hit
GGGCAAGGATTACATCGCCCTGAACGAGGACCTGCGCTCCTGGACCGCCG340.11296806990729973No Hit
GGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT340.11296806990729973No Hit
GGGCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGC330.10964547961590856No Hit
GGGATTCTCTTTTCTTTTATTCTTGTCTGTTCTGCCTCACTCCCGAGCTC330.10964547961590856No Hit
GGGATTCTCCCCAGAGGCCGAGATGCGGGTCATGGCGCCCCGAGCCCTCC320.10632288932451739No Hit
GGGGCCCTATAATTTGGAATGTGGGTGGAGAGGGGTCATAGTTCTCCCTG310.10300029903312623No Hit
GGGACTTGGGTTCATGGTCTCTAATATTTCAAACAGGAGCTCCCTTTAGC310.10300029903312623No Hit
GGGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCATCCTGCTG310.10300029903312623No Hit
GGGCCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAG310.10300029903312623No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)