FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8759
Sequences flagged as poor quality0
Sequence length36-200
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGGTTATAGAAGTATCTGTCCAGGAACCGCACCCGCTCCGTCCCA480.5480077634433155No Hit
GCGCTAGGCCGCCCCAGCTCCGTCACCGCCCGGTACTCCCCCACGTCGCT330.37675533736727934No Hit
GCGTCAGGCCGCCCCAGCTCCGTCACCGCCCGGTACTCCCCCACGTCGCT300.34250485215207216No Hit
GCCACCGGGGTGCTCAGCATCGCCAGCATCAAGGTCACAGTTGCTACCCG280.319671195341934No Hit
GCGCTAGGCCGCCCCTGCGGCGTCACCGCCCGGTACACCCCCACGTCGCT240.27400388172165774No Hit
GCACCGTGAAGCTCTCACCAACCCCGTAGTTGTGTCTGCAGTAGGTGTCC210.23975339650645053No Hit
GTCACGGCCTCGTCCAGCTCGTAGTTGTGTCTGCATACCCTGTCCGGCAC160.18266925448110516No Hit
GCACATCCTGTCCGGCACTGCCCGCTTCTCCTCCAGGATGTCCTTCTGGC160.18266925448110516No Hit
CTCCACAGCCCCGTAGTTGTGTCTGCAGTAGGTGTCCACCGCGGCCCGCG160.18266925448110516No Hit
GTTCATACCCGCGGAGGAAGCGCCTGTCGGGCCCCAGCTCGCAGCCATGC160.18266925448110516No Hit
CCGCCTGGTTATAGAAGTATCTGTCCAGGAACCGCACCCGCTCCGTCCCA150.17125242607603608No Hit
GCGCGAGGCCGCCCCAGCTCCGTCACCGCCCGGTACTCCCCCACGTCGCT140.159835597670967No Hit
GGACTTCCTTCTGGCTGTTCCAGTACTCGGCGCTAGGCCGCCCCTGCGGC140.159835597670967No Hit
GCCACCGGGGTGCTCAGGATCGCCAGCATCAAGGTGACAGTTGCTACCCG130.14841876926589792No Hit
TAGATGCCTCTGTGCAGGAACCGCACCCGCTCCGTCCCGTTGAAGAAATG130.14841876926589792No Hit
GTCCTCATCAGGCCGCCCCAGCTCCGTCACCGCCCGGAACTCCCCCACGT120.13700194086082887No Hit
GGTCTGGTGTCCCCAGCCAAAGCCAGTGGGGAGCTCAGCACCATCAGTGT110.1255851124557598No Hit
CTGCACATCCTGTCCGGCACTGCCCGCTTCTCCTCCAGGATGTCCTTCTG110.1255851124557598No Hit
GCATACCCTGTCCGGCACTGCCCGCTTCTCCTCCAGGAGGTCCTTCTGGC110.1255851124557598No Hit
GGCTCAACTCGCCGCTGCACTGTGAAGCTCTCCACAGCCCCGTAGTTGTG110.1255851124557598No Hit
GCGCTAGGCCGCCCCAGCGGCGTCACCGCCCGATACACCCCCACGTCGCT110.1255851124557598No Hit
CACGTTCTCCTCTTGGTTATAGATGCCTCTGTGCAGGAACCGCACCCGCT100.11416828405069071No Hit
GGACGTCCTTCTGGCTGTTCCAGTACTCGGCGCTAGGCCGCCCCTGCGGC100.11416828405069071No Hit
GCGGCAGGCCGCCCCAGCTCCGTCACCGCCCGGTACTCCCCCACGTCGCT100.11416828405069071No Hit
GCACATCCTGTCCGGCACTGCCCGCTTCTCCTCCAGGAGGTCCTTCTGGC100.11416828405069071No Hit
GAGACGGGATGGTGGGTCACGTGTGTCTTTGGGTGTTCCGCGCGCTGCAG100.11416828405069071No Hit
CCTCTTGGTTATAGAAGTATCTGTCCAGGAACCGCACCCGCTCCGTCCCA100.11416828405069071No Hit
GGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGTTTCTATTTCCTGA90.10275145564562163No Hit
GCCCCGTAGTTGTGTCTGCAGTAGGTGTCCACCGCGGCCCGCGCCTGCTC90.10275145564562163No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)