FastQCFastQC Report
Tue 7 May 2024
EGAF00004963758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23000
Total Bases2 Mbp
Sequences flagged as poor quality0
Sequence length36-90
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGT7413.2217391304347824No Hit
GGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGAC4652.0217391304347827No Hit
GGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACTC3201.391304347826087No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTG3191.3869565217391304No Hit
GGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCC3081.3391304347826087No Hit
GGGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTC2971.2913043478260868No Hit
GGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCCA2901.2608695652173914No Hit
GGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCC2821.2260869565217392No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA2531.0999999999999999No Hit
GGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCATCCTGCTGC2481.0782608695652174No Hit
GGGAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTG2040.8869565217391304No Hit
GGGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACT2030.8826086956521739No Hit
GGGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTC2010.8739130434782608No Hit
GGGAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTG1980.8608695652173913No Hit
GGGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCC1860.808695652173913No Hit
GGGGAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCT1850.8043478260869565No Hit
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT1850.8043478260869565No Hit
GGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCAG1820.791304347826087No Hit
GGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGC1700.7391304347826086No Hit
GGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCG1610.7000000000000001No Hit
GGGGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTGT1430.6217391304347827No Hit
GGGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCATCCTGCTG1400.6086956521739131No Hit
GGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAACA1380.6No Hit
GGGGAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCT1370.5956521739130435No Hit
GGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCC1250.5434782608695652No Hit
GGGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA1150.5No Hit
GGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCTG1130.491304347826087No Hit
GGGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTC1120.4869565217391304No Hit
GGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA1100.47826086956521735No Hit
GGGCCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCT940.408695652173913No Hit
GGGGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTGT920.4No Hit
GGGGGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTG910.3956521739130435No Hit
GGGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAC890.3869565217391304No Hit
GGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGG870.37826086956521743No Hit
GGGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCT770.3347826086956522No Hit
GGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTG750.32608695652173914No Hit
GGGGCAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCTC730.3173913043478261No Hit
GGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGC710.30869565217391304No Hit
GGGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTT630.27391304347826084No Hit
GGGCAAGGATTACATCGCCCTGAACGAGGATCTGCGCTCCTGGACCGCCG600.26086956521739135No Hit
GGGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCC590.25652173913043474No Hit
GGGGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTGTCCTGTTCTC580.25217391304347825No Hit
GGGGGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTGTCCTGTTCT580.25217391304347825No Hit
GGGGGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTG580.25217391304347825No Hit
GGGCGCCTCCTCCGCGGGTATAACCAGTTCGCCTACGACGGCAAGGATTA560.2434782608695652No Hit
GGGGCCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTC550.23913043478260868No Hit
GGGCCATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTTCGTCT520.2260869565217391No Hit
GGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA500.21739130434782608No Hit
GGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG500.21739130434782608No Hit
GGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG490.21304347826086958No Hit
GGGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCC460.2No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTC450.1956521739130435No Hit
GGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAG450.1956521739130435No Hit
GGGAGATCCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTC440.19130434782608696No Hit
GGGATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTCA430.18695652173913044No Hit
GGGGAGATCCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTT430.18695652173913044No Hit
GGGAGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCT430.18695652173913044No Hit
GGGCTCACAATTACTCTACAGCTCAGAACACCAACTGCTGAGGCTGCCTT410.1782608695652174No Hit
GGGCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGC400.17391304347826086No Hit
GGGCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGC400.17391304347826086No Hit
GGGGAGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCC380.16521739130434784No Hit
GGGAGATTCTCCCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACCC360.1565217391304348No Hit
GGGCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTC350.15217391304347827No Hit
GGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCG350.15217391304347827No Hit
GGGATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTTCGTCTCA340.14782608695652175No Hit
GGGGGCAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCT340.14782608695652175No Hit
GGGGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCTGTCCTCTTCTC330.14347826086956522No Hit
GGGCCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAG330.14347826086956522No Hit
GGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCTGAGCTGTGTTGACT320.1391304347826087No Hit
GGGGCCATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTTCGTC310.13478260869565217No Hit
GGGGGACTCAGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCG300.13043478260869568No Hit
GGGACTCCCGAGCTCTACTGACTCCCAACAGAGCGCCCAAGAAGAAAATG300.13043478260869568No Hit
GGGGAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTT300.13043478260869568No Hit
GGGACTCAGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAA290.12608695652173912No Hit
GGGGGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCTGTCCTCTTCT290.12608695652173912No Hit
GGGATTCTCTTTTCTTTTATTCTTGTCTGTTCTGCCTCACTCCCGAGCTC280.1217391304347826No Hit
GGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAA280.1217391304347826No Hit
GGGAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCTCCA270.11739130434782609No Hit
GGGGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCCAG260.11304347826086955No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG260.11304347826086955No Hit
GGGAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTGCTTTTCCCTTC260.11304347826086955No Hit
GGGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCTGAGCTGTGTTGAC250.10869565217391304No Hit
GGGACAGCAGTCCGTGCCGCCGTCCCGCCCGCCAGCGCCCCAGCGAGGAA250.10869565217391304No Hit
GGGGGAATGTGGGTGGAGGGGTTCATAGTTCTCCCTGAGTGAGACTTGCC240.10434782608695652No Hit
GGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTT240.10434782608695652No Hit
GGGAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCTG240.10434782608695652No Hit
GGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCT240.10434782608695652No Hit
GGGCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACT240.10434782608695652No Hit
GGGGTCCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGA240.10434782608695652No Hit
GGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG240.10434782608695652No Hit
GGGTCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTT240.10434782608695652No Hit
GGGCGCTTCCTCCGCGGGTACCACCAGTACGCCTACGACGGCAAGGATTA240.10434782608695652No Hit
GGGGAGATTCTCCCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACC240.10434782608695652No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)