FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23000
Sequences flagged as poor quality0
Sequence length36-200
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTCAACTCGCCGCTGCACTGTGAAGCTCTCACCAACCCCGTAGTTGTG800.34782608695652173No Hit
CACGTTCTCCTCCTGGTTATGGAAGTATCTGTCCAGGAACCGCACCCGCT610.26521739130434785No Hit
CTCGTCTTCCAGGATGTCCTTCTGGCTGTTCCAGTACTCGGCATCAGGCC550.23913043478260868No Hit
GGGCCCGTTGGGAGGCCCAGCGGGCAGGAGGAACGGCTACCGAGGCTCCA460.2No Hit
CACGGTCTCCTCCTGGTTATGGAAGTATCTGTCCAGGAACCGCACCCGCT450.1956521739130435No Hit
GGGCCCGTTGGGAGGCCCAAGGGGCAAGAAGCATGGCCACCGAGGCTCCA340.14782608695652175No Hit
GTAATTGTCCACCTGGCCCCGCTTCTGCTCCAGGAGGTCCTTCTGGCTGT340.14782608695652175No Hit
GGCCCCCTCCAGGACTTCCTTCTGGCTGTTCCAGTACTCGGCAACAGGCC320.1391304347826087No Hit
CGCGGCTTCCAGGATGTCCTTCTGGCTGTTCCAGTACTCGGCATCAGGCC290.12608695652173912No Hit
GCACATCCTGTCCGGCACTGCCCGCTTCTCCTCCAGGATGTCCTTCTGGC280.1217391304347826No Hit
CCCTTAACCTGGGCAGAGCCGTGGCTCAGGTCGAAGTGCGGGAAGTAGGT270.11739130434782609No Hit
GCATACCCTGTCCGGCACTGCCCGCTTCTCCTCCAGGAGGTCCTTCTGGC260.11304347826086955No Hit
CCACCGCGGCCCGCCTCTGCTCCAGGAGGTCCTTCTGGCTGTTCCAGTAC250.10869565217391304No Hit
TGGAAGTATCTGTCCAGGAACCGCACCCGCTCCGTCCCATTGAAGAAATG250.10869565217391304No Hit
GGTACAGGAAGTAGGCGGTGGTGGCCTGGCCAGCGAGGAGCAGAGTCACC240.10434782608695652No Hit
GGTCATACCCGCGGAGGAGGCGCCCGTCGGGCCCCACGTCGCAGCCATAC240.10434782608695652No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)