FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004963766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217405
Sequences flagged as poor quality0
Sequence length36-90
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGC34571.5901198224511854No Hit
GGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGG19660.9043030289091788No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGT16000.735953634921No Hit
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT14420.6632782134725512No Hit
GGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGAC13350.6140613141372093No Hit
GGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCG12980.5970423863296612No Hit
GGGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCC12860.5915227340677538No Hit
GGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG12440.5722039511510775No Hit
GGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCCA11190.5147075734228744No Hit
GGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCG10160.467330558174835No Hit
GGGCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTTCTGG10040.4618109059129275No Hit
GGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA8510.3914353395736069No Hit
GGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAG8440.3882155424208275No Hit
GGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAA8260.37993606402796626No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA8160.37533635380971No Hit
GGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCA8050.3702766725696281No Hit
GGGCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACT7860.36153722315494125No Hit
GGGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCC7850.3610772521331156No Hit
GGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGC7770.3573974839585106No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTG7660.35233780271842874No Hit
GGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG7580.34865803454382377No Hit
GGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG7340.33761873002000875No Hit
GGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTT7300.33577884593270624No Hit
GGGGCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTTCTG7270.3343989328672294No Hit
GGGGCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAAC5910.27184287389894435No Hit
GGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT5450.2506842068949656No Hit
GGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGC5030.23136542397828938No Hit
GGGAGCCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTTC4960.22814562682550998No Hit
GGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACAGTGC4200.1931878291667625No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4170.1918079161012856No Hit
GGGGCCAGCGGCGGAGTCTCCAACTGGGAGAGCTGCAGCTGCCGAGAGGA4150.19088797405763439No Hit
GGGCAGTTGACACAAAATAGACTACGAAAGTGGCTTTAACATATCTGAAC4130.18996803201398313No Hit
GGGACAAACTTTCAGAGACAGCAGAGCACACAAGCTTCTAGGACAAGAGC4030.18536832179572688No Hit
GGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACA3900.17938869851199374No Hit
GGGTGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT3750.17248913318460937No Hit
GGGACAGCAGTCCGTGCCGCCGTCCCGCCCGCCAGCGCCCCAGCGAGGAA3600.165589567857225No Hit
GGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCA3510.16144982866079438No Hit
GGGGAGCCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTT3440.15823003150801498No Hit
GGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAGGCCTC3440.15823003150801498No Hit
GGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATG3390.15593017639888687No Hit
GGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGA3340.15363032128975876No Hit
GGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACTC3330.15317035026793313No Hit
GGGCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGCACCTC3180.14627078494054874No Hit
GGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCTGGAGTT3160.1453508428968975No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT3050.14029116165681563No Hit
GGGGACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTC2880.13247165428578No Hit
GGGCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAG2830.13017179917665186No Hit
GGGGGCCAGCGGCGGAGTCTCCAACTGGGAGAGCTGCAGCTGCCGAGAGG2820.12971182815482624No Hit
GGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCTGGAGT2800.12879188611117498No Hit
GGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACG2770.12741197304569812No Hit
GGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCTGGTGC2760.1269520020238725No Hit
GGGGGAGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGT2750.12649203100204687No Hit
GGGGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACC2740.12603205998022127No Hit
GGGGCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGCACCT2730.12557208895839564No Hit
GGGGACAAACTTTCAGAGACAGCAGAGCACACAAGCTTCTAGGACAAGAG2670.12281226282744187No Hit
GGGGACAGCAGTCCGTGCCGCCGTCCCGCCCGCCAGCGCCCCAGCGAGGA2670.12281226282744187No Hit
GGGAGTGTGAAATCTTCAGAGAAGAATTTCTCTTTAGTTCTTTGCAAGAA2590.11913249465283689No Hit
GGGGCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAA2570.11821255260918563No Hit
GGGCTCTTTCCTTGCCTAACGCAGCCATGGCTCGTGGTCCCAAGAAGCAT2550.11729261056553437No Hit
GGGGGACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACT2350.10809319012902187No Hit
GGGATGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT2340.10763321910719624No Hit
GGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGAC2230.10257353786711437No Hit
GGGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACT2210.10165359582346312No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)