FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963770

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963770
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55100
Sequences flagged as poor quality0
Sequence length36-90
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGC7011.2722323049001816No Hit
GGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCG4380.79491833030853No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGT4060.7368421052631579No Hit
GGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGG4010.7277676950998185No Hit
GGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA3970.720508166969147No Hit
GGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGAC3650.662431941923775No Hit
GGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG3260.5916515426497277No Hit
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT3150.5716878402903811No Hit
GGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCCA2980.5408348457350273No Hit
GGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGC2860.5190562613430127No Hit
GGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG2610.4736842105263158No Hit
GGGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCC2510.45553539019963707No Hit
GGGCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACT2360.42831215970961883No Hit
GGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCG2150.3901996370235935No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA2110.38294010889292196No Hit
GGGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCC2100.38112522686025413No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTG1910.34664246823956446No Hit
GGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGC1880.3411978221415608No Hit
GGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTT1780.32304900181488205No Hit
GGGCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTTCTGG1750.3176043557168784No Hit
GGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAG1700.308529945553539No Hit
GGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCA1680.30490018148820325No Hit
GGGGCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAAC1660.30127041742286753No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1620.29401088929219604No Hit
GGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGGAG1400.2540834845735027No Hit
GGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGGA1370.24863883847549909No Hit
GGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGA1370.24863883847549909No Hit
GGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCTGGAGTT1290.23411978221415608No Hit
GGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAA1150.2087114337568058No Hit
GGGCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAG1120.20326678765880218No Hit
GGGGCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTTCTG1120.20326678765880218No Hit
GGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACG1110.2014519056261343No Hit
GGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAGGCCTC1100.1996370235934664No Hit
GGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG1080.19600725952813067No Hit
GGGTGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACTT950.1724137931034483No Hit
GGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCTGGTGC940.1705989110707804No Hit
GGGCTCTTTCCTTGCCTAACGCAGCCATGGCTCGTGGTCCCAAGAAGCAT940.1705989110707804No Hit
GGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCTGGAGT920.16696914700544463No Hit
GGGGCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAA890.16152450090744103No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT840.15245009074410162No Hit
GGGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCTGGTG820.14882032667876588No Hit
GGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATG810.14700544464609802No Hit
GGGCTTTCCAACTTGGACGCTGCAGAATGGCTCCCGCAAAGAAGGGTGGC790.14337568058076225No Hit
GGGAGCCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTTC770.1397459165154265No Hit
GGGCTCTCGGCCTTAGCGCCATTTTTTTGGAAACCTCTGCGCCATGAGAG760.13793103448275862No Hit
GGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT740.13430127041742287No Hit
GGGCCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGC740.13430127041742287No Hit
GGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCA730.132486388384755No Hit
GGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACTC720.13067150635208713No Hit
GGGCCTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGCTA710.12885662431941924No Hit
GGGCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGCACCTC710.12885662431941924No Hit
GGGGCTCTTTCCTTGCCTAACGCAGCCATGGCTCGTGGTCCCAAGAAGCA710.12885662431941924No Hit
GGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAGGCCT700.12704174228675136No Hit
GGGCTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTT700.12704174228675136No Hit
GGGCTTTTCACAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCCT690.1252268602540835No Hit
GGGAACTCGGTGGTGGCCACTACGCAGACCAGACTTCGCTCGTACTCGTG660.11978221415607986No Hit
GGGATGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCTCACT660.11978221415607986No Hit
GGGCTCTTTCCCATCTTGCAAGATGGCGGGTGAAAAAGTTGAGAAGCCAG640.11615245009074411No Hit
GGGCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGCG620.11252268602540835No Hit
GGGCCTTTCCGTCTGGCGGCAGCCATCAGGTAAGCCAAGATGGGTGCATA610.11070780399274048No Hit
GGGCTTTTTCGTCTGGGCTGCCAACATGCCATCCAGACTGAGGAAGACCC600.10889292196007261No Hit
GGGGCTTTCCAACTTGGACGCTGCAGAATGGCTCCCGCAAAGAAGGGTGG600.10889292196007261No Hit
GGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGAC590.10707803992740471No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)