FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004963776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences524071
Sequences flagged as poor quality0
Sequence length36-90
%GC59

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGT180043.4354123773305525No Hit
GGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGAC144942.7656557985463803No Hit
GGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG90121.7196143270663706No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA80551.5370054820816264No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTG75171.4343476361027419No Hit
GGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGC68241.3021136449068924No Hit
GGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGG34820.6644137912611078No Hit
GGGCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACT32090.6123216129112277No Hit
GGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG31920.609077777629367No Hit
GGGGCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAAC23080.4403983429726125No Hit
GGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCCA22760.4342923000891101No Hit
GGGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCC22360.426659746484732No Hit
GGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCG19770.37723896189638423No Hit
GGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG18960.3617830408475188No Hit
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT18890.3604473439667526No Hit
GGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCG18540.3537688595629218No Hit
GGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA16950.32342945898551917No Hit
GGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTT16850.3215213205844246No Hit
GGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAG15440.29461656912899203No Hit
GGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAA13510.25778949798786804No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT13430.2562629872669924No Hit
GGGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCC13410.25588135958677355No Hit
GGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAACCA12270.23412858181429616No Hit
GGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT11680.22287056524783858No Hit
GGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACAGTGC11570.2207716130066346No Hit
GGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGC9950.18985977090890357No Hit
GGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGC9620.1835629141852917No Hit
GGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGA9150.1745946637001475No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8060.15379595512821737No Hit
GGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCTGGAGTT7750.1478807260848244No Hit
GGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCTGGTGC7580.1446368908029637No Hit
GGGACCGCCGAGACCGCGTCCGCCCCGCGAGCACAGAGCCTCGCCTTTGC7400.1412022416809936No Hit
GGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACG7390.14101142784088416No Hit
GGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCATG7100.1354778264777101No Hit
GGGCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGCACCTC7070.13490538495738175No Hit
GGGGACAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTC6700.12784527287333206No Hit
GGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAGGCCTC6610.126127948312347No Hit
GGGCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAG6550.12498306527169029No Hit
GGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCTGGAGT6510.12421980991125248No Hit
GGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACTC6480.12364736839092413No Hit
GGGCCTTTCCGTCTGGCGGCAGCCATCAGGTAAGCCAAGATGGGTGCATA6440.12288411303048632No Hit
GGGATTGCAGACTGCGGAGTCAGACGGCGCTATGTACGCCCTCTTCCTCC6410.12231167151015797No Hit
GGGCTCTTTCCTTGCCTAACGCAGCCATGGCTCGTGGTCCCAAGAAGCAT6280.11983109158873512No Hit
GGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCCCCA6210.11849539470796895No Hit
GGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTACACA6100.116396442466765No Hit
GGGGCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAA6100.116396442466765No Hit
GGGCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGCG6050.11544237326621773No Hit
GGGAGTGTGAAATCTTCAGAGAAGAATTTCTCTTTAGTTCTTTGCAAGAA5600.10685575046129245No Hit
GGGACAGCAGTCCGTGCCGCCGTCCCGCCCGCCAGCGCCCCAGCGAGGAA5570.10628330894096412No Hit
GGGCCCTTTTGGCTCTCTGACCAGCACCATGGCGGTTGGCAAGAACAAGC5460.10418435669976014No Hit
GGGATTCTTCCCCTCTCTACAACCCTCTCTCCTCAGCGCTTCTTCTTTCT5390.10284865981899399No Hit
GGGGCCTTTCCGTCTGGCGGCAGCCATCAGGTAAGCCAAGATGGGTGCAT5250.10017726605746169No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)