FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13628
Sequences flagged as poor quality0
Sequence length36-90
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGT2681.9665394775462284No Hit
GGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCC1971.4455532726739067No Hit
GGGAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTG1841.3501614323451716No Hit
GGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGAC1761.2914587613736423No Hit
GGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCG1701.2474317581449956No Hit
GGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACTC1561.1447020839448196No Hit
GGGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTC1551.1373642500733785No Hit
GGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGC1270.9319049016730261No Hit
GGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGC1070.7851482242442032No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTG1060.7778103903727619No Hit
GGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCCA1040.7631347226298797No Hit
GGGGAGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCT1040.7631347226298797No Hit
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT930.682418550044027No Hit
GGGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACT920.6750807161725858No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA890.6530672145582623No Hit
GGGGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTGT820.6017023774581743No Hit
GGGCCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCT820.6017023774581743No Hit
GGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCAG780.5723510419724098No Hit
GGGGGTTCTCCCTGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTG680.49897270325799825No Hit
GGGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCC680.49897270325799825No Hit
GGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGG680.49897270325799825No Hit
GGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCCTCCTGCTGC670.4916348693865571No Hit
GGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCATCCTGCTGC670.4916348693865571No Hit
GGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA630.46228353390079246No Hit
GGGGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCTC600.44027003228646905No Hit
GGGGGGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCC590.43293219841502795No Hit
GGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCTG570.4182565306721456No Hit
GGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCC570.4182565306721456No Hit
GGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA560.4109186968007044No Hit
GGGGGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCT550.40358086292926326No Hit
GGGGCCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTC540.39624302905782216No Hit
GGGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA520.38156736131493985No Hit
GGGGGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCTGTCCTGTTCT510.3742295274434987No Hit
GGGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCATCCTGCTG500.36689169357205753No Hit
GGGCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTC460.3375403580862929No Hit
GGGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTC460.3375403580862929No Hit
GGGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCT450.3302025242148518No Hit
GGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAA450.3302025242148518No Hit
GGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG410.30085118872908717No Hit
GGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTG410.30085118872908717No Hit
GGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCG410.30085118872908717No Hit
GGGATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTCA410.30085118872908717No Hit
GGGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCC410.30085118872908717No Hit
GGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAAA400.293513354857646No Hit
GGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAG390.2861755209862049No Hit
GGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTT380.2788376871147637No Hit
GGGAGAGTCTCCTCAGACGCCAAGATGCTGGTCATGGCGCCCCGAACCGT380.2788376871147637No Hit
GGGGGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTGTCCTGTTCT380.2788376871147637No Hit
GGGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGC370.2714998532433226No Hit
GGGGCAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCTC370.2714998532433226No Hit
GGGGAGTGAGACTTGCCTGCTTCTCTGGCCCCTGGTCCTGTCCTGTTCTC360.26416201937188144No Hit
GGGAGATCCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTC350.2568241855004402No Hit
GGGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCC340.24948635162899913No Hit
GGGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCCTCCTGCTG330.24214851775755797No Hit
GGGATAATTTGGAATGTGGGTGGAGGGGGATCATAGTTCTCCCTGAGTGA330.24214851775755797No Hit
GGGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTT320.2348106838861168No Hit
GGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCTGAGCTGTGTTGACT310.22747285001467565No Hit
GGGGATAATTTGGAATGTGGGTGGAGGGGGATCATAGTTCTCCCTGAGTG310.22747285001467565No Hit
GGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCTGGAGT310.22747285001467565No Hit
GGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCTGGAGTT310.22747285001467565No Hit
GGGGAGATCCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTT310.22747285001467565No Hit
GGGCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGCACCTC300.22013501614323452No Hit
GGGCCCTTTCATCAGTCTTCCCGCGTCCGCCGATTCCTCCTCCTTGGTCG290.21279718227179334No Hit
GGGAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCTG290.21279718227179334No Hit
GGGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAA290.21279718227179334No Hit
GGGAGATTCTCCCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACCC290.21279718227179334No Hit
GGGGAGAGTCTCCTCAGACGCCAAGATGCTGGTCATGGCGCCCCGAACCG280.2054593484003522No Hit
GGGGCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCT270.19812151452891108No Hit
GGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACGA250.18344584678602877No Hit
GGGCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAACT250.18344584678602877No Hit
GGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG250.18344584678602877No Hit
GGGGAGATTCTCCCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACC250.18344584678602877No Hit
GGGGCTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGG230.16877017904314645No Hit
GGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGTG230.16877017904314645No Hit
GGGGCCCTTTCATCAGTCTTCCCGCGTCCGCCGATTCCTCCTCCTTGGTC230.16877017904314645No Hit
GGGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCTGAGCTGTGTTGAC220.1614323451717053No Hit
GGGCCCCATAATTTGGAATGTGGGTGGAGGGGGATCATAGTTCTCCCTGA220.1614323451717053No Hit
GGGGAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCT210.15409451130026416No Hit
GGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATGACG210.15409451130026416No Hit
GGGCTCTTTTCCGTGGCGCCTCGGAGGCGTTCAGCTGCTTCAAGATGAAG210.15409451130026416No Hit
GGGATCATAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGG200.146756677428823No Hit
GGGGGGGCCCGACGGGCGCCTCCTCCGCGGGTATAACCAGTTCGCCTACG200.146756677428823No Hit
GGGGAACTCGGTGGTGGCCACTGCGCAGACCAGACTTCGCTCGTACTCGT200.146756677428823No Hit
GGGAGTAACTTCCTCCCTATAACTTGGAATGTGGGTGGAGGGGTTCATAG200.146756677428823No Hit
GGGGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTC200.146756677428823No Hit
GGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCTGGTGC190.13941884355738185No Hit
GGGACATCAGATCCATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTC190.13941884355738185No Hit
GGGAGACGCCAAGATGCTGGTCATGGCGCCCCGAACCGTCCTCCTGCTGC190.13941884355738185No Hit
GGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCG190.13941884355738185No Hit
GGGGATCAGGTCTGAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTC180.13208100968594072No Hit
GGGCCTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGCTA180.13208100968594072No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTC180.13208100968594072No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGA180.13208100968594072No Hit
GGGAGGGGCGGTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTGTGGGT180.13208100968594072No Hit
GGGGCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTGTCGTGAAAAC170.12474317581449956No Hit
GGGGGACTCGGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCG170.12474317581449956No Hit
GGGCTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTCGCCATGGGC170.12474317581449956No Hit
GGGCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGC170.12474317581449956No Hit
GGGCTCTACCGGCGGGATTTGATGGCGTGATGTCTCACAGAAAGTTCTCC170.12474317581449956No Hit
GGGGAGTCTCCTCAGACGCCAAGATGCTGGTCATGGCGCCCCGAACCGTC170.12474317581449956No Hit
GGGCTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTTT170.12474317581449956No Hit
GGGCTTTCCGCTCGGCTGTTTTCCTGCGCAGGAGCCGCAGGGCCGTAGGC170.12474317581449956No Hit
GGGCTCTTTCCTTGCCTAACGCAGCCATGGCTCGTGGTCCCAAGAAGCAT170.12474317581449956No Hit
GGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAGGCCTC160.1174053419430584No Hit
GGGCAAGGATTACATCGCCCTGAACGAGGACCTGCGCTCCTGGACCGCCG160.1174053419430584No Hit
GGGGCTCTTTCCGGTGTGGAGTCTGGAGACGACGTGCAGAAATGGCACCT160.1174053419430584No Hit
GGGCTCACAATTACTCTACAGCTCAGAACACCAACTGCTGAGGCTGCCTT160.1174053419430584No Hit
GGGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCT150.11006750807161726No Hit
GGGGCCTTTCCTCAGCTGCCGCCAAGGTGCTCGGTCCTTCCGAGGAAGCT150.11006750807161726No Hit
GGGGATCATAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTG150.11006750807161726No Hit
GGGGGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCCTG140.1027296742001761No Hit
GGGCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCTGTGCTCACAGTCA140.1027296742001761No Hit
GGGGCTTCTCTGGCCCCTGGTCCTGTCCTGTTCTCCAGCATGGTGTGTCT140.1027296742001761No Hit
GGGCTTTTCACAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTGCCCCT140.1027296742001761No Hit
GGGGCTTTTCCAAGCGGCTGCCGAAGATGGCGGAGGTGCAGGTCCTGGTG140.1027296742001761No Hit
GGGGCTCTTTCCTTGCCTAACGCAGCCATGGCTCGTGGTCCCAAGAAGCA140.1027296742001761No Hit
GGGGCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATGAAGGCCT140.1027296742001761No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)