FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004963788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004963788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13471
Sequences flagged as poor quality0
Sequence length36-90
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCC5313.9418009056491723No Hit
GGGAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTG4633.437012842402197No Hit
GGGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTC3782.8060277633434785No Hit
GGGGAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCT3022.2418528691262716No Hit
GGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTGT2862.1230792071858064No Hit
GGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACTC2281.692524682651622No Hit
GGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCCA2191.6257144978101106No Hit
GGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGAC1661.2322767426323213No Hit
GGGGAGGACTCAGAGGCTGGGATCATGGTAGATGGAACCCTCCTTTTACT1531.1357731423056938No Hit
GGGGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTGT1461.0838096652067404No Hit
GGGGAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCC1411.0466928958503452No Hit
GGGGACTCTTCTGGTCCCCACAGACTCAGAGAGAACCCACCATGGTGCTG1220.9056491722960432No Hit
GGGGACATTTGCTTCTGACACAACTGTGTTCACTAGCAACCTCAAACAGA1160.8611090490683692No Hit
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT990.7349120332566254No Hit
GGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGGC920.682948556157672No Hit
GGGGGTTCTCCCTGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTG910.675525202286393No Hit
GGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAACA780.5790216019597654No Hit
GGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCTG620.4602479400193007No Hit
GGGGAGTCTCATCTGCCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCT620.4602479400193007No Hit
GGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGCG560.4157078167916265No Hit
GGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCAG550.4082844629203474No Hit
GGGGCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA530.39343775517778934No Hit
GGGGATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCCCAAC530.39343775517778934No Hit
GGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTG530.39343775517778934No Hit
GGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCCA500.37116769356395224No Hit
GGGGCAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCTC490.36374433969267317No Hit
GGGAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTTTTCCCTTCGTC460.341474278078836No Hit
GGGGGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTGTCCTGTTCT430.3192042164649989No Hit
GGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCGTGG410.30435750872244083No Hit
GGGCGCCTCCTCCGCGGGTATAACCAGTTCGCCTACGACGGCAAGGATTA380.2820874471086037No Hit
GGGGAGATCCATCAGGTCCGAGCTGTGTTGACTACCACTTTTCCCTTCGT360.2672407393660456No Hit
GGGAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCGTCCTCCTGCTGC360.2672407393660456No Hit
GGGAGAGTCTCCTCAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCGT340.2523940316234875No Hit
GGGGAGTGAGACTTGCCTGCTGCTCTGGCCCCTGGTCCTGTCCTGTTCTC330.24497067775220843No Hit
GGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTC330.24497067775220843No Hit
GGGCAAGGATTACATCGCCCTGAACGAGGATCTGCGCTCCTGGACCGCCG310.23012397000965035No Hit
GGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTCC310.23012397000965035No Hit
GGGAGATTCTCCCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACCC300.22270061613837133No Hit
GGGGCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGCCATCCTTTTCC300.22270061613837133No Hit
GGGCCTTTCCGGCGGTGACGACCTACGCACACGAGAACATGCCTCTCGCA290.2152772622670923No Hit
GGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTTG280.20785390839581325No Hit
GGGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCATCCTGCTG280.20785390839581325No Hit
GGGATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTCA280.20785390839581325No Hit
GGGGCTCTGGCCCCTGGTCCTGTCCTGTTCTCCAGCATGGTGTGTCTGAG270.2004305545245342No Hit
GGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGGC260.19300720065325513No Hit
GGGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCC250.18558384678197612No Hit
GGGAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCTCATCCTGCTGC250.18558384678197612No Hit
GGGGGGGTGGAGGGGTTCATAGTTCTCCCTGAGTGAGACTTGCCTGCTGC240.17816049291069705No Hit
GGGGCCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTT240.17816049291069705No Hit
GGGAGATCCATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTC240.17816049291069705No Hit
GGGAGTAACTTACTCCCTATAACTTGGAATGTGGGTGGAGGGGTTCATAG220.16331378516813896No Hit
GGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAG220.16331378516813896No Hit
GGGGAGATTCTCCCCAGACGCCGAGGATGGCCGTCATGGCGCCCCGAACC220.16331378516813896No Hit
GGGGATAGTTCTCCCTGAGTGAGACTTGCCTGCTCCTCTGGCCCCTGGTC210.15589043129685992No Hit
GGGTCTTTTATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTC210.15589043129685992No Hit
GGGGAGATCCATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTT210.15589043129685992No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGA200.14846707742558088No Hit
GGGATTCTCTTTTCTTTTATTCTTGTCTGTTCTGCCTCACTCCCGAGCTC200.14846707742558088No Hit
GGGGGTGGAGGGGTTCATAGTTCTCCCTGAGTGAGACTTGCCTGCTGCTC190.14104372355430184No Hit
GGGCCTTTAGCGAGTCCTTCTTTTCCTGACTGCAGCTCTTTTCATTTTGC190.14104372355430184No Hit
GGGGCCCTTTCATCAGTCTTCCCGCGTCCGCCGATTCCTCCTCCTTGGTC190.14104372355430184No Hit
GGGGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGCCAG190.14104372355430184No Hit
GGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCCAAGCTGTGTTGACT190.14104372355430184No Hit
GGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAA190.14104372355430184No Hit
GGGCCATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCT180.1336203696830228No Hit
GGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCTGGAGTT180.1336203696830228No Hit
GGGGCTGCTCTGGCCCCTGGTCCTGTCCTGTTCTCCAGCATGGTGTGTCT180.1336203696830228No Hit
GGGGCCATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTC180.1336203696830228No Hit
GGGGGCAGACACTGACCTTCAGCGCCTCGGCTCCAGCGCCATGGCGCCCT180.1336203696830228No Hit
GGGCAAGGATTACATCGCCCTGAAAGAGGACCTGCGCTCTTGGACCGCGG180.1336203696830228No Hit
GGGGGGCATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCG180.1336203696830228No Hit
GGGCATCAGGTCCAAGCTGTGTTGACTACCACTACTTTTCCCTTCGTCTC180.1336203696830228No Hit
GGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTT170.12619701581174375No Hit
GGGGAGTAACTTACTCCCTATAACTTGGAATGTGGGTGGAGGGGTTCATA170.12619701581174375No Hit
GGGGTCCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGA170.12619701581174375No Hit
GGGCTCACAATTGCTCTACAGCTCAGAACAGCAACTGCTGAGGCTGCCTT170.12619701581174375No Hit
GGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAATTAATTAACCA170.12619701581174375No Hit
GGGCTTTTATTCTTGTCTGTTCTGCCTCACTCCCGAGCTCTACTGACTCC170.12619701581174375No Hit
GGGACTCCCGAGCTCTACTGACTCCCAACAGAGCGCCCAAGAAGAAAATG170.12619701581174375No Hit
GGGGCTTTCTGCCAGGTACATCAGATCCATCAGGTCCAAGCTGTGTTGAC170.12619701581174375No Hit
GGGGGTCCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAG160.1187736619404647No Hit
GGGACTTGGGTTCATGGTCTCTAATATTTCAAACAGGAGCTCCCTTTAGC160.1187736619404647No Hit
GGGACTCGGCCTCAGTTCCTCATCACTGTTCCTGTGCTCACAGTCATCAA160.1187736619404647No Hit
GGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAG150.11135030806918567No Hit
GGGGCTCTTCCGGGGACGTTGTCTGCAGGCACTCAGAATGGTCCAGCGTT150.11135030806918567No Hit
GGGGGGTGGAGGGGTTCATAGTTCTCCCTGAGTGAGACTTGCCTGCTGCT150.11135030806918567No Hit
GGGGAGTCTCCTCAGACGCCGAGATGCTGGTCATGGCGCCCCGAACCGTC150.11135030806918567No Hit
GGGAGAATCTCCTCAGACGCCGAGATGCGGGTCACGGCGCCCCGAACCGT150.11135030806918567No Hit
GGGCTCCACTCGGCCTCAGTTCCTCATCACTGTTCCTGTGCTCACAGTCA140.10392695419790662No Hit
GGGGCCCTTTCCCCTGGCTGGCAGCGCGGAGGCCGCACGATGCCTGGAGT140.10392695419790662No Hit
GGGCCAGATGCACAGGAGGAGAAGCAGGAGCTGTCGGGAAGATCAGAAGC140.10392695419790662No Hit
GGGAGATTCTCCCCAGACGCCGAGATGCGGGTCATGGCGCCCCGAACCCT140.10392695419790662No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)